School of Biomedical Science, Faculty of Medicine, University of Queensland, Brisbane, Queensland, Australia.
Genomics Research Centre, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, Australia.
Methods Mol Biol. 2020;2129:217-240. doi: 10.1007/978-1-0716-0377-2_17.
Technological advances in the form of next-generation sequencing allow sequencing of large numbers of different DNA sequences in a single/parallel reaction compared to conventional sequencing. It is a powerful tool which has enabled comprehensive characterization of esophageal squamous cell carcinoma. Whole-genome sequencing is the most comprehensive but expensive, whereas whole-exome sequencing is cost-effective, but it only works for the known genes. Thus, second-generation sequencing methods can provide a complete picture of the esophageal squamous cell carcinoma genome by detecting and discovering different type of alterations in the cancer which may lead to the development of effective diagnostic and therapeutic approaches for esophageal squamous cell carcinoma.
下一代测序技术的进步使得在单个/平行反应中可以对大量不同的 DNA 序列进行测序，这与传统测序相比是一个巨大的进步。它是一种强大的工具，使食管鳞癌的全面特征得以实现。全基因组测序是最全面的，但也是最昂贵的，而全外显子组测序则具有成本效益，但它仅适用于已知基因。因此，第二代测序方法可以通过检测和发现癌症中不同类型的改变，为食管鳞癌基因组提供一个完整的画面，这可能为食管鳞癌的有效诊断和治疗方法的发展提供线索。
