College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China; Zhejiang A&F University, Hangzhou, Zhejiang, 311300, China.
Biosens Bioelectron. 2020 Apr 15;154:112036. doi: 10.1016/j.bios.2020.112036. Epub 2020 Jan 22.
Biosensors contribute a lot to the reliable and sensitive detection in various fields, especially emerging trends of in-field and real-time detection for point-of-care diagnosis, food safety and environmental monitoring. The signal amplification that improves the analytical performance and the compact integration of various biosensing components with/in miniaturized and portable devices are essential but still challenging. Integrating the merits of bio-active proteins (enzyme, antibody, etc.) and nanomaterials (nanoparticles, nanotubes, nanosheets, nanoflowers etc.) with abundant physicochemical properties, numerous protein-nanomaterial hybrids (PN hybrids) have been designed and applied for biosensing in recent years. PN hybrids can serve as not only sensitive probes for analyte recognition and signal generation/amplification thereby enhancing analytical performance, but also miniaturized and full-functional sensing components that are easily combined with other devices, greatly simplifying the construction and assay procedures. In this review, the state-of-art strategies of PN hybrids for biosensing are summarized from the view of the role of nanomaterial components, i.e. immobilization matrix, catalyst, and label. Recent advances for the emerging in-field detection applications of PN hybrids with the incorporation of portable hand-held readers and miniaturized devices are then surveyed. The features of PN hybrids for the construction of these miniaturized biosensors are focused. The integration and synergy between proteins and nanomaterials for biosensing is emphasized and discussed.
生物传感器在各个领域的可靠和敏感检测中发挥了重要作用，特别是在即时和现场检测方面，用于即时诊断、食品安全和环境监测。信号放大可以提高分析性能，将各种生物传感组件与/或在小型化和便携式设备中的紧凑集成是必不可少的，但仍然具有挑战性。生物活性蛋白（酶、抗体等）和纳米材料（纳米粒子、纳米管、纳米片、纳米花等）具有丰富的物理化学性质，将它们的优点与生物传感器相结合，近年来已经设计并应用了许多蛋白质-纳米材料杂化（PN 杂化）用于生物传感。PN 杂化不仅可以作为分析物识别和信号产生/放大的敏感探针，从而提高分析性能，还可以作为小型化的全功能传感组件，与其他设备易于结合，大大简化了构建和分析程序。在这篇综述中，从纳米材料组件（即固定基质、催化剂和标记物）的角度总结了 PN 杂化用于生物传感的最新策略。然后调查了结合便携式手持读取器和小型化设备的 PN 杂化在新兴现场检测应用方面的最新进展。重点介绍了 PN 杂化用于构建这些小型化生物传感器的特点。强调并讨论了蛋白质和纳米材料在生物传感中的集成和协同作用。
