College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Int J Biol Macromol. 2020 May 15;151:595-601. doi: 10.1016/j.ijbiomac.2020.02.049. Epub 2020 Feb 10.
The aim of this study was to investigate the protective effect of Polygonatum sibiricum polysaccharides (PSP) on acute kidney injury (AKI) in rats. The AKI model was established with 100 mg/kg of gentamicin (GM) intramuscularly. Six hours post-injection, rats were given intragastrically different dosages of PSP (0.25, 0.5, and 1 g/kg) for seven consecutive days. Results showed that compared with the model group, the kidney coefficient of rats decreased significantly (P < 0.05), and rat serum CRE and urea levels were significantly decreased (P < 0.05) in the PSP treatment group. The expression of NGAL, KIM-1, IL-1β, IL-6, TNF-α, and p38 MAPK mRNA in the kidney were significantly decreased (P < 0.05). The protein expression of p38 MAPK, p-p38 MAPK, and p-ATF2 were decreased to different degrees. Among the three-PSP treatment groups, the medium-dose group displayed the most protective effects. In conclusion, PSP had a powerful protective effect on GM-induced AKI in rats, it decreased the expression of NGAL or KIM-1 mRNA in kidney tissue and inhibited the p38 MAPK/ATF2 signaling pathway and the production of inflammatory factors TNF-α, IL-1β, and IL-6.
本研究旨在探讨黄精多糖（PSP）对大鼠急性肾损伤（AKI）的保护作用。采用 100mg/kg 庆大霉素（GM）肌肉注射建立 AKI 模型。注射后 6 小时，大鼠连续 7 天给予不同剂量的 PSP（0.25、0.5 和 1g/kg）灌胃。结果表明，与模型组相比，PSP 治疗组大鼠的肾脏系数显著降低（P<0.05），血清 CRE 和尿素水平显著降低（P<0.05）。PSP 治疗组大鼠肾脏中 NGAL、KIM-1、IL-1β、IL-6、TNF-α和 p38 MAPK mRNA 的表达显著降低（P<0.05）。p38 MAPK、p-p38 MAPK 和 p-ATF2 的蛋白表达均有不同程度的降低。在 PSP 三个治疗组中，中剂量组的保护作用最强。综上所述，PSP 对 GM 诱导的大鼠 AKI 具有强大的保护作用，可降低肾脏组织中 NGAL 或 KIM-1 mRNA 的表达，并抑制 p38 MAPK/ATF2 信号通路和 TNF-α、IL-1β 和 IL-6 等炎症因子的产生。
