State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.
State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China; The Institute for Advanced Study, Nanchang University, Nanchang 330006, China.
Int J Biol Macromol. 2024 Oct;277(Pt 2):132290. doi: 10.1016/j.ijbiomac.2024.132290. Epub 2024 May 23.
Polygonatum cyrtonema Hua (PC) with different processing degrees during the nine-steam-nine-bask processing was selected as the research object to investigate the changes of polysaccharide structure and their protective effect on cisplatin-induced acute kidney injury (AKI) in mice. The polysaccharides (PCP0, PCP4 and PCP9) were extracted, whose polysaccharide contents were 62.45 %, 60.34 % and 58.23 %, respectively. After processing, the apparent structure of PCPs became looser, and the apparent viscosity and the particle size were decreased. The PCPs were acidic polysaccharides containing pyran rings, and furan rings were present in PCP4 and PCP9. Besides, processing destroyed the original β-glucoside bond in PCP0. PCPs were all composed of Rha, Man, Glu, Gal, Xyl and Ara with different ratio. In addition, AKI mice model was successfully constructed by single intraperitoneal injection of 15 mg/kg cisplatin. PC extracts (3.0750 g/kg) and PCP (0.1599 g/kg) significantly decreased the kidney function, liver function, and percentage of renal cell apoptosis, and improved the kidney structure of AKI mice (p < 0.05). PC and PCP have protective effect on cisplatin-induced AKI mice, and the protective effect was improved with the increase of processing degree. Under the same processing degree, the protective effect of PC mixed extract was better than that of PCP.
选用经过九蒸九晒炮制的不同程度的玉竹（Polygonatum cyrtonema Hua，PC）作为研究对象，研究多糖结构的变化及其对顺铂诱导的急性肾损伤（AKI）小鼠的保护作用。提取多糖（PCP0、PCP4 和 PCP9），其多糖含量分别为 62.45%、60.34%和 58.23%。炮制后，PCP 的表观结构变得更加疏松，表观粘度和粒径减小。PCPs 是含有吡喃环的酸性多糖，PCP4 和 PCP9 中存在呋喃环。此外，炮制破坏了 PCP0 中原有的β-葡萄糖苷键。PCPs 均由不同比例的 Rha、Man、Glu、Gal、Xyl 和 Ara 组成。此外，通过单次腹腔注射 15mg/kg 顺铂成功构建 AKI 小鼠模型。PC 提取物（3.0750g/kg）和 PCP（0.1599g/kg）显著降低了肾功能、肝功能和肾细胞凋亡率，并改善了 AKI 小鼠的肾脏结构（p<0.05）。PC 和 PCP 对顺铂诱导的 AKI 小鼠具有保护作用，且随着炮制程度的增加保护作用增强。在相同的炮制程度下，PC 混合提取物的保护作用优于 PCP。
