Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, College of Pharmacy, Shihezi University, Shihezi 832002, China.
Shihezi Institute for Drug Control, Shihezi 832002, China.
Molecules. 2022 Oct 21;27(20):7121. doi: 10.3390/molecules27207121.
The clinical application of gentamicin may lead to acute kidney injury (AKI), and the nephrotoxicity of gentamicin is related to the pathological mechanism of several oxidative and inflammatory cytokines. Plant-derived essential oils have good anti-inflammatory and antioxidant properties. This study aimed to clarify the protective effect of essential oils (AOs) on gentamicin-induced AKI in rats and its possible mechanism. The rat AKI model was induced by intraperitoneal injection of gentamicin. After 14 days of oral AO treatment, the renal function and pathological changes of the kidney tissues were evaluated, and the level of kidney tissue oxidative stress was detected. The content of inflammatory cytokines was measured by ELISA. The expression of ERK1/2, JNK1/2, p38, NF-κB, caspase-3, and Bax/Bcl-2 proteins were estimated by Western blot analysis. The results showed that taking AO reduced the contents of serum urea and creatinine in AKI rats and improve the pathological changes and oxidative stress of the kidney tissue in rats. At the same time, AO reduced inflammation and apoptosis during AKI by regulating the MAPK pathway. The data show that AO has a protective effect on the kidneys and may be a potential drug for treating kidney injury.
庆大霉素的临床应用可能导致急性肾损伤（AKI），而庆大霉素的肾毒性与几种氧化和炎症细胞因子的病理机制有关。植物源性精油具有良好的抗炎和抗氧化特性。本研究旨在阐明精油（AOs）对庆大霉素诱导的大鼠 AKI 的保护作用及其可能的机制。通过腹腔注射庆大霉素诱导大鼠 AKI 模型。经过 14 天的口服 AO 治疗，评估肾脏功能和肾组织的病理变化，并检测肾脏组织的氧化应激水平。通过 ELISA 测定炎症细胞因子的含量。通过 Western blot 分析评估 ERK1/2、JNK1/2、p38、NF-κB、caspase-3 和 Bax/Bcl-2 蛋白的表达。结果表明，AO 降低了 AKI 大鼠血清尿素和肌酐的含量，并改善了大鼠肾组织的病理变化和氧化应激。同时，AO 通过调节 MAPK 通路减少 AKI 期间的炎症和细胞凋亡。数据表明，AO 对肾脏具有保护作用，可能是治疗肾损伤的潜在药物。
