Seri-biotech Research Laboratory, Central Silk Board, Kodathi, Carmelaram Post, Kodathi, Bengaluru 560 035, India.
Seri-biotech Research Laboratory, Central Silk Board, Kodathi, Carmelaram Post, Kodathi, Bengaluru 560 035, India..
Comp Biochem Physiol B Biochem Mol Biol. 2020 Apr;242:110417. doi: 10.1016/j.cbpb.2020.110417. Epub 2020 Feb 11.
The vitellogenin receptor (VgR) plays a critical role in egg development by mediating endocytosis of the major yolk protein precursor vitellogenin (Vg). Therefore, identifying the VgR of beneficial insects and its characterization could lead to the development of novel egg production strategies to enhance their commercial values. Here, we present the cloning, expression, and functional characterization of the VgR from an economically important eri silkworm, Samia ricini. The complete mRNA sequence was 6002 bp with an ORF of 5484 bp, encoding a protein of 1827 amino acids. Sequence analyses revealed that the SrVgR contained all of the conservative structural motifs characteristics of LDLR family members. The SrVgR was specifically expressed in the ovary, and the mRNA level increased steadily in pupal stages, reached its peak on day 9, and then declined to a bare minimum in adults. RNA interference (RNAi) clearly reduced the VgR transcript levels, disrupted the ovarian development resulting in malformed ovarioles and abnormal development of eggs. Taken together, these data provide conclusive evidence for the essential roles of VgR in insect reproduction.
卵黄蛋白原受体（VgR）在卵发育过程中通过介导主要卵黄蛋白前体卵黄蛋白原（Vg）的内吞作用发挥关键作用。因此，鉴定有益昆虫的 VgR 及其特征可能会导致开发新的产卵策略来提高其商业价值。在这里，我们介绍了一种经济上重要的野桑蚕，桑蚕，卵黄蛋白原受体（VgR）的克隆、表达和功能特征。完整的 mRNA 序列为 6002bp，ORF 为 5484bp，编码 1827 个氨基酸的蛋白质。序列分析表明，SrVgR 包含 LDLR 家族成员的所有保守结构基序特征。SrVgR 特异性表达于卵巢，mRNA 水平在蛹期稳步增加，在第 9 天达到峰值，然后在成虫中降至最低。RNA 干扰（RNAi）明显降低了 VgR 转录本水平，破坏了卵巢发育，导致卵巢小管畸形和卵子发育异常。总之，这些数据为 VgR 在昆虫生殖中的重要作用提供了确凿的证据。
