Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Fish Nutrition and Safety in Production Sichuan University Key Laboratory, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Key Laboratory of Animal Disease-resistant Nutrition, Sichuan Province, China.
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Fish Shellfish Immunol. 2020 Apr;99:243-256. doi: 10.1016/j.fsi.2020.02.019. Epub 2020 Feb 11.
To investigate effects of vitamin A (VA) on fish immune function and structural integrity in the head kidney and spleen of fish, total of 540 on-growing grass carp (Ctenopharyngodon idella) were divided into six groups, feeding graded levels of VA (0, 600, 1200, 1800, 2800 and 3800 IU/kg diet) for 70 days. Results showed that dietary VA deficiency depressed antibacterial ability and aggravated inflammatory response partially linked to nuclear factor κB p65 (NF-κB p65) and target of rapamycin (TOR) signaling pathways in the head kidney and spleen of fish. Meanwhile, VA deficiency caused oxidative damage, apoptosis and disruption of tight junctions (TJs), which were partially attributed to the down-regulation of NF-E2-related factor 2 (Nrf2) signaling mediated antioxidant ability, the up-regulation of p38 mitogen-activated protein kinase (p38MAPK) signaling mediated apoptosis and myosin light chain kinase (MLCK) signaling mediated disruption of tight junctions (TJs). Taken together, current study firstly demonstrated that VA deficiency decreased the immune function and damaged the structural integrity of the head kidney and spleen in fish.
为了研究维生素 A（VA）对鱼类免疫功能和头肾、脾脏结构完整性的影响，将 540 尾草鱼（Ctenopharyngodon idella）分为 6 组，分别投喂不同 VA 水平（0、600、1200、1800、2800 和 3800IU/kg 饲料）的饲料，为期 70 天。结果表明，VA 缺乏会降低鱼类的抗菌能力，并部分加剧头肾和脾脏的炎症反应，这与核因子 κB p65（NF-κB p65）和雷帕霉素靶蛋白（TOR）信号通路有关。同时，VA 缺乏会导致氧化损伤、细胞凋亡和紧密连接（TJs）破坏，这部分归因于 NF-E2 相关因子 2（Nrf2）信号介导的抗氧化能力下调、p38 丝裂原活化蛋白激酶（p38MAPK）信号介导的细胞凋亡和肌球蛋白轻链激酶（MLCK）信号介导的 TJs 破坏。总之，本研究首次表明，VA 缺乏会降低鱼类头肾和脾脏的免疫功能并破坏其结构完整性。
