Department of Chemistry, University of Toronto, Toronto, ON, Canada; School of the Environment, University of Toronto, Toronto, ON, Canada.
Department of Chemistry, University of Toronto, Toronto, ON, Canada; School of the Environment, University of Toronto, Toronto, ON, Canada.
Comp Biochem Physiol Part D Genomics Proteomics. 2020 Jun;34:100655. doi: 10.1016/j.cbd.2020.100655. Epub 2020 Jan 29.
Current environmental toxicological studies are challenged by the immense number (>80,000) of chemical contaminants. While several strategies including adverse outcome pathway (AOP), High-Throughput Screening (HTS), and computational toxicology have been proposed to tackle this challenge, we argue here that characterizing the protein targets of chemical contaminants is the major bottleneck. In this commentary article, we reviewed current environmental toxicology research, and pinpointed the urgency to identify physical protein targets of chemical contaminants. We also reviewed several chemical proteomics methodologies developed in our and other groups, and their advantages and disadvantages to identify protein targets. At the end of the article, we also pointed out several potential follow-up research directions should be pursued once protein targets are identified.
当前的环境毒理学研究面临着巨大数量（>80,000）的化学污染物的挑战。虽然已经提出了几种策略，包括不良结局途径（AOP）、高通量筛选（HTS）和计算毒理学，来应对这一挑战，但我们在这里认为，化学污染物的蛋白质靶标的特征描述是主要的瓶颈。在这篇评论文章中，我们回顾了当前的环境毒理学研究，并指出了确定化学污染物的物理蛋白质靶标的紧迫性。我们还回顾了我们和其他小组开发的几种化学蛋白质组学方法及其优缺点，以确定蛋白质靶标。在文章的最后，我们还指出了一旦确定了蛋白质靶标，应该跟进的几个潜在的后续研究方向。
