Environmental Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, 793022, India.
Environmental Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, 793022, India.
Environ Toxicol Pharmacol. 2020 May;76:103347. doi: 10.1016/j.etap.2020.103347. Epub 2020 Jan 30.
Alpha-cypermethrin (ACy) is a synthetic pyrethroid insecticide commonly used in agricultural practices for controlling a broad range of insect pests particularly belonging to the order Lepidoptera and Coleoptera. The present study aims to evaluate the toxic effect of ACy on microalgae by studying its influence on Chlorella sp. According to our knowledge, this is the first detailed study of ACy toxicity on microalgae. Significant growth inhibition of Chlorella sp. was observed at high ACy concentration (6-48 mg L) during the entire 96 h bioassay. The 96 h median effective concentration (EC) of ACy was estimated to be 11.00 mg L. Flow cytometry analysis showed an enhanced generation of reactive oxygen species (ROS) and intracellular lipid accumulation after 96 h exposure to 11.00 mg L of ACy. Further, the same ACy concentration showed a significant decrease in photosynthetic pigment content and an increase in antioxidant enzyme activity and malondialdehyde (MDA) content in Chlorella sp.
α-氯氰菊酯（ACy）是一种常用于农业实践中的合成拟除虫菊酯杀虫剂，用于防治鳞翅目和鞘翅目等多种害虫。本研究旨在通过研究其对小球藻的影响来评估 ACy 对微藻的毒性作用。据我们所知，这是首次详细研究 ACy 对微藻的毒性。在整个 96 小时生物测定中，高浓度 ACy（6-48mg/L）对小球藻的生长有显著抑制作用。ACy 的 96 小时半数有效浓度（EC）估计为 11.00mg/L。流式细胞术分析显示，在 11.00mg/L ACy 暴露 96 小时后，活性氧（ROS）的产生和细胞内脂质积累增加。此外，相同浓度的 ACy 使小球藻中的光合色素含量显著降低，抗氧化酶活性和丙二醛（MDA）含量增加。
