Opt Lett. 2020 Feb 15;45(4):865-868. doi: 10.1364/OL.380032.
A large field-of-view and fast scanning of photoacoustic microscopy (PAM) relatively have been difficult to obtain due to the water-drowned structure of the system for the transmission of ultrasonic signals. Researchers have widely studied the achievement of a waterproof scanner for dynamic biological applications with a high-resolution and high signal-to-noise ratio. This Letter reports a novel, to the best of our knowledge, waterproof galvanometer scanner-based PAM system with a successfully attainable ${9.0};{\rm mm} \times {14.5};{\rm mm}$9.0mm×14.5mm scan region, amplitude scan rate of 40 kHz, and spatial resolution of 4.9 µm. The in vivo characterization of a mouse brain in intact-skull microvascular visualization demonstrated its capability in biomedical imaging and is anticipated to be an effective technique for various preclinical and clinical studies.
由于超声信号传输系统的浸水结构，大视场和快速扫描的光声显微镜（PAM）相对较难实现。研究人员广泛研究了具有高分辨率和高信噪比的防水扫描仪在动态生物应用中的实现。本研究报告了一种新颖的、据我们所知的基于防水振镜的 PAM 系统，该系统可实现成功达到的${9.0};{\rm mm} \times {14.5};{\rm mm}$9.0mm×14.5mm 扫描区域、40 kHz 的振幅扫描速率和 4.9 µm 的空间分辨率。在完整颅骨微血管可视化的小鼠大脑的体内特征表明，它具有在生物医学成像中的应用潜力，有望成为各种临床前和临床研究的有效技术。
