Kim Jin Young, Lee Changho, Park Kyungjin, Lim Geunbae, Kim Chulhong
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea.
Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea.
Sensors (Basel). 2015 Apr 27;15(5):9815-26. doi: 10.3390/s150509815.
Optical-resolution photoacoustic microscopy (OR-PAM) is an imaging tool to provide in vivo optically sensitive images in biomedical research. To achieve a small size, fast imaging speed, wide scan range, and high signal-to-noise ratios (SNRs) in a water environment, we introduce a polydimethylsiloxane (PDMS)-based 2-axis scanner for a flexible and waterproof structure. The design, theoretical background, fabrication process and performance of the scanner are explained in details. The designed and fabricated scanner has dimensions of 15 × 15 × 15 mm along the X, Y and Z axes, respectively. The characteristics of the scanner are tested under DC and AC conditions. By pairing with electromagnetic forces, the maximum scanning angles in air and water are 18° and 13° along the X and Y axes, respectively. The measured resonance frequencies in air and water are 60 and 45 Hz along the X axis and 45 and 30 Hz along the Y axis, respectively. Finally, OR-PAM with high SNRs is demonstrated using the fabricated scanner, and the PA images of micro-patterned samples and microvasculatures of a mouse ear are successfully obtained with high-resolution and wide-field of view. OR-PAM equipped with the 2-axis PDMS based waterproof scanner has lateral and axial resolutions of 3.6 μm and 26 μm, respectively. This compact OR-PAM system could potentially and widely be used in preclinical and clinical applications.
光学分辨率光声显微镜（OR-PAM）是一种在生物医学研究中用于提供体内光学敏感图像的成像工具。为了在水环境中实现小尺寸、快速成像速度、宽扫描范围和高信噪比（SNR），我们引入了一种基于聚二甲基硅氧烷（PDMS）的两轴扫描仪，以实现灵活且防水的结构。详细解释了该扫描仪的设计、理论背景、制造工艺和性能。所设计和制造的扫描仪在X、Y和Z轴方向上的尺寸分别为15×15×15毫米。在直流和交流条件下测试了该扫描仪的特性。通过与电磁力配合，在空气中和水中沿X和Y轴的最大扫描角度分别为18°和13°。在空气中和水中沿X轴测得的共振频率分别为60赫兹和45赫兹，沿Y轴分别为45赫兹和30赫兹。最后，使用制造的扫描仪展示了具有高信噪比的OR-PAM，并成功获得了微图案样品和小鼠耳部微血管的高分辨率和宽视野光声图像。配备基于两轴PDMS的防水扫描仪的OR-PAM的横向和轴向分辨率分别为3.6微米和26微米。这种紧凑的OR-PAM系统有可能广泛应用于临床前和临床应用。
