State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
Bioresour Technol. 2021 Feb;322:124555. doi: 10.1016/j.biortech.2020.124555. Epub 2020 Dec 16.
A bacterial strain was isolated and identified as Pseudomonas sp. DM02 from an aquaculture system. Strain DM02 showed efficient heterotrophic nitrification-aerobic denitrification capability. Total ammonia nitrogen (TAN, 10 mg/L) could be completely removed by strain DM02 within 12 h under low nutrient condition. Nitrogen mass balance indicated that 70.8% of the initial TAN was translated into gaseous nitrogen and 28.1% was converted into intracellular nitrogen. Various carbon sources can be used for nitrate removal (>95% within 28 h). The optimal conditions for TAN, nitrate and nitrite removal were pH 7 with carbon/nitrogen (C/N) ratios of 8, 12 and 12, respectively. The napA, nirK, and nosZ functional genes were successful amplified from strain DM02. Both bioaugmentation and immobilized technology of strain DM02 present ability (>88%) for continuous treatment of real aquaculture wastewater. This research indicated a great potential for practical application of Pseudomonas sp. DM02 in aquaculture wastewater treatment.
从水产养殖系统中分离并鉴定出一种名为 Pseudomonas sp. DM02 的细菌菌株。该菌株 DM02 表现出高效的异养硝化-好氧反硝化能力。在低营养条件下，菌株 DM02 可在 12 小时内完全去除总氨氮（TAN，10mg/L）。氮质量平衡表明，初始 TAN 的 70.8%转化为气态氮，28.1%转化为细胞内氮。各种碳源可用于去除硝酸盐（28 小时内>95%）。TAN、硝酸盐和亚硝酸盐去除的最佳条件分别为 pH 7 和碳氮比（C/N）为 8、12 和 12。成功从菌株 DM02 中扩增出 napA、nirK 和 nosZ 功能基因。菌株 DM02 的生物强化和固定化技术都具有连续处理实际水产养殖废水的能力（>88%）。本研究表明，Pseudomonas sp. DM02 在水产养殖废水处理中的实际应用具有很大的潜力。
