Morejón Alonso Lizette, Fernández Torres Iran, Zayas Tamayo Ángela M, Ledea Lozano Oscar Ernesto, Durán Ramos Ivette, Delgado García-Menocal José Ángel, Rios-Donato Nely, Mendizábal Eduardo
Center of Biomaterials, University of Havana, Havana, Cuba.
National Center for Scientific Research, Havana, Cuba.
J Infect Dev Ctries. 2019 Jun 30;13(6):487-495. doi: 10.3855/jidc.10716.
Antibiotic-loaded bone cements of poly(methyl methacrylate) are considered as very useful biomaterials for the management of corporal deep osseous infections. However, the high prevalence of resistant germs and polymicrobial infections makes it necessary to search for new formulations of bone cements containing antibiotics for local antibacterial therapy. In this work, bone cements loaded with drugs with different mechanism of action were evaluated to determine its antibacterial effectiveness on Pseudomonas aeruginosa.
Poly(methyl methacrylate) cements loaded with 10 wt.% of Oleozon®, mixtures of Ciprofloxacin/Meropenem and Ciprofloxacin/Meropenem/Oleozon® were prepared. The in vitro drugs release in water was followed by UV-Vis spectroscopy, and their antibacterial activity against Pseudomonas aeruginosa was evaluated for 11 days using the microdilution method.
All the extracts demonstrated an inhibitory effect on the growth of the strain during the whole trial period. Extracts from cement with Oleozon® only presented a total antibacterial inhibitory effect during 20 hours for the extracts taken at day 1 while the extracts from the cements loaded with mixtures of Ciprofloxacin/Meropenem and Ciprofloxacin/Meropenem/Oleozon® showed complete inhibition of the growth of the microorganism, even at 11 days. At the end of the trial period, some of the drugs remained inside the matrices, indicating that they can be released for a longer time in treatments.
The results indicated a positive antibacterial effect by the combined used of the two or the three drugs tested against the Gram-negative bacilli Pseudomonas aeruginosa, so these proposal may be a valid alternative to be considered by surgeons.
聚甲基丙烯酸甲酯载抗生素骨水泥被认为是治疗深部骨感染的非常有用的生物材料。然而，耐药菌的高流行率和混合感染使得有必要寻找含抗生素骨水泥的新配方用于局部抗菌治疗。在本研究中，对载有不同作用机制药物的骨水泥进行评估，以确定其对铜绿假单胞菌的抗菌效果。
制备了含10 wt.%奥乐松、环丙沙星/美罗培南混合物以及环丙沙星/美罗培南/奥乐松的聚甲基丙烯酸甲酯骨水泥。通过紫外可见光谱法跟踪其在水中的体外药物释放，并使用微量稀释法评估其对铜绿假单胞菌11天的抗菌活性。
在整个试验期间，所有提取物均对菌株生长表现出抑制作用。仅含奥乐松的骨水泥提取物在第1天取样时，在20小时内呈现出完全的抗菌抑制作用，而载有环丙沙星/美罗培南混合物以及环丙沙星/美罗培南/奥乐松的骨水泥提取物即使在11天时仍显示出对微生物生长的完全抑制。在试验期结束时，一些药物仍留在基质中，表明它们在治疗中可长时间释放。
结果表明，所测试的两种或三种药物联合使用对革兰氏阴性杆菌铜绿假单胞菌具有积极的抗菌作用，因此这些提议可能是外科医生可考虑的有效替代方案。
