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通过环介导等温扩增（LAMP）检测波兰野生鸟类中的禽腺病毒 D 株。

Detection of fowl adenovirus D strains in wild birds in Poland by Loop-Mediated Isothermal Amplification (LAMP).

机构信息

Department of Poultry Diseases, National Veterinary Research Institute, Partyzantow 57, 24-100, Pulawy, Poland.

出版信息

BMC Vet Res. 2020 Feb 14;16(1):58. doi: 10.1186/s12917-020-2271-4.

DOI:10.1186/s12917-020-2271-4
PMID:32059679
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7023798/
Abstract

BACKGROUND

The present study on the role of strains of adenovirus in wildlife reservoirs, and their prevalence is under exploration. In several previous studies, the presence of adenovirus strains in wild birds has been investigated. Worldwide distribution and outbreaks of adenovirus infections have been reported by many authors. The present study investigated the prevalence of FAdVs in 317 samples of different bird species from the northwestern region of Poland. An applied specific, sensitive, and efficient, without cross-reactivity loop-mediated isothermal amplification (LAMP) method to gauge the prevalence of fowl adenovirus strains in wild birds was developed and used.

RESULTS

The method was based on the sequence of the loop L1 HVR1-4 region of the hexon gene of the FAdV genome reference strains FAdV-2 KT862805 (ANJ02325), FAdV-3 KT862807 (ANJ02399) and FAdV-11 KC750784 (AGK29904). The results obtained by LAMP were confirmed by real-time PCR. Among 317 samples obtained from wild birds, eight FAdV isolates (2.52%) were identified and produced a cytopathic effect (CPE) in chicken embryo kidney cells (CEK). Three FAdV types belonging to species Fowl adenovirus D were detected, which were isolated from three adenovirus types 2/3/11, and have been confirmed in three mute swans (Cygnus olor), three wild ducks (Anas platyrhynchos), one owl (Strigiformes), and one common wood pigeon (Columba palumbus).

CONCLUSIONS

This study provides the first accurate quantitative data for the replication of fowl adenovirus strains in wild birds in Poland, indicating adenovirus interspecies transmission, and demonstrating the circulation of FAdVs in wild birds.

摘要

背景

目前，人们正在探索腺病毒株在野生动物宿主中的作用及其流行情况。在之前的几项研究中，已经调查了野生鸟类中腺病毒株的存在。许多作者报告了腺病毒感染的全球分布和暴发。本研究调查了波兰西北部 317 种不同鸟类样本中 FAdVs 的流行情况。开发并应用了一种应用特异性、敏感性和高效的，无交叉反应的环介导等温扩增（LAMP）方法来衡量野生鸟类中禽腺病毒株的流行情况。

结果

该方法基于 FAdV 基因组参考株 FAdV-2 KT862805（ANJ02325）、FAdV-3 KT862807（ANJ02399）和 FAdV-11 KC750784（AGK29904）的 hexon 基因环 L1 HVR1-4 区的序列。通过 LAMP 获得的结果通过实时 PCR 得到证实。从野生鸟类中获得的 317 个样本中，鉴定出 8 个 FAdV 分离株（2.52%），并在鸡胚肾细胞（CEK）中产生细胞病变效应（CPE）。检测到三种属于 Fowl adenovirus D 的 FAdV 型，它们从三种腺病毒 2/3/11 型中分离出来，并在三只默西鹅（Cygnus olor）、三只野鸭（Anas platyrhynchos）、一只猫头鹰（Strigiformes）和一只普通斑鸠（Columba palumbus）中得到证实。

结论

本研究首次提供了波兰野生鸟类中禽腺病毒株复制的准确定量数据，表明了腺病毒种间传播，并证明了 FAdVs 在野生鸟类中的循环。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/1ad9db364391/12917_2020_2271_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/4c2ec2f7efeb/12917_2020_2271_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/64c6d88a7419/12917_2020_2271_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/cffb139794ec/12917_2020_2271_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/32d2e6148d7d/12917_2020_2271_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/075a67289b9a/12917_2020_2271_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/1ad9db364391/12917_2020_2271_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/4c2ec2f7efeb/12917_2020_2271_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/64c6d88a7419/12917_2020_2271_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/cffb139794ec/12917_2020_2271_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/32d2e6148d7d/12917_2020_2271_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/075a67289b9a/12917_2020_2271_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2781/7023798/1ad9db364391/12917_2020_2271_Fig6_HTML.jpg

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