Zentrum für Molekulare Biologie, Universität Heidelberg, DKFZ-ZMBH Allianz, 69120, Heidelberg, Germany.
Electron Microscopy Core Facility, Universität Heidelberg, 69120, Heidelberg, Germany.
Nat Commun. 2020 Feb 14;11(1):903. doi: 10.1038/s41467-020-14767-2.
Centrosomes are essential organelles with functions in microtubule organization that duplicate once per cell cycle. The first step of centrosome duplication is the daughter centriole formation followed by the pericentriolar material recruitment to this centriole. This maturation step was termed centriole-to-centrosome conversion. It was proposed that CEP295-dependent recruitment of pericentriolar proteins drives centriole conversion. Here we show, based on the analysis of proteins that promote centriole biogenesis, that the developing centriole structure helps drive centriole conversion. Depletion of the luminal centriole protein CEP44 that binds to the A-microtubules and interacts with POC1B affecting centriole structure and centriole conversion, despite CEP295 binding to centrioles. Impairment of POC1B, TUBE1 or TUBD1, which disturbs integrity of centriole microtubules, also prevents centriole-to-centrosome conversion. We propose that the CEP295, CEP44, POC1B, TUBE1 and TUBD1 centriole biogenesis pathway that functions in the centriole lumen and on the cytoplasmic side is essential for the centriole-to-centrosome conversion.
中心体是具有微管组织功能的必需细胞器，在细胞周期中复制一次。中心体复制的第一步是子中心粒的形成，随后是周围物质向该中心粒募集。这个成熟步骤被称为中心粒到中心体的转化。有人提出，CEP295 依赖性募集周围蛋白驱动中心粒转化。在这里，我们基于促进中心粒发生的蛋白质的分析表明，正在发育的中心粒结构有助于驱动中心粒转化。尽管 CEP295 与中心粒结合，但耗尽结合 A 微管并与 POC1B 相互作用影响中心粒结构和中心粒转化的腔中心粒蛋白 CEP44，会抑制中心粒转化。破坏 POC1B、TUBE1 或 TUBD1，它们会干扰中心粒微管的完整性，也会阻止中心粒到中心体的转化。我们提出，CEP295、CEP44、POC1B、TUBE1 和 TUBD1 中心粒发生途径在中心粒腔和细胞质侧发挥作用，对于中心粒到中心体的转化是必需的。
