生物源银纳米颗粒可降低刚地弓形虫RH株在HeLa细胞中的黏附、感染及增殖，且不会诱导炎症介质产生。

Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction.

作者信息

Machado Laís Fernanda, Sanfelice Raquel Arruda, Bosqui Larissa Rodrigues, Assolini João Paulo, Scandorieiro Sara, Navarro Italmar Teodorico, Depieri Cataneo Allan Henrique, Wowk Pryscilla Fanini, Nakazato Gerson, Bordignon Juliano, Pavanelli Wander Rogerio, Conchon-Costa Ivete, Costa Idessania Nazareth

机构信息

Departamento de Patologia Experimental - Laboratório de Imunoparasitologia Das Doenças Negligenciadas e Câncer. Universidade Estadual de Londrina, 86057-970, Londrina, Paraná, Brazil.

Departamento de Microbiologia - Laboratório de Bacteriologia Básica e Aplicada. Universidade Estadual de Londrina, Paraná, Brazil.

出版信息

Exp Parasitol. 2020 Feb 17;211:107853. doi: 10.1016/j.exppara.2020.107853.

DOI:10.1016/j.exppara.2020.107853

PMID:32061628

Abstract

The highlights of biogenic silver nanoparticles (AgNp-Bio) include low toxicity - depending on size and concentration - and efficient antiparasitic activity. Therefore, the objective of this study was to assess the action of the AgNp-Bio on HeLa cells in an infection with strain of RH Toxoplasma gondii. Firstly, we performed a cellular viability test and characterized the AgNp-Bio to proceed with the infection of HeLa cells with T. gondii to be treated using AgNp-Bio or conventional drugs. Subsequently, we determined the level of standard cytokines Th1/Th2 as well as the content of nitric oxide (NO) and reactive oxygen species (ROS). Results indicated a mean size of 69 nm in diameter for the AgNp-Bio and obtained a dose-dependent toxicity. In addition, the concentrations of 3 and 6 μM promoted a significant decrease in adherence, infection, and intracellular proliferation. We also found lower IL-8 and production of inflammatory mediators. Thus, the nanoparticles reduced the adherence, infection, and proliferation of ROS and NO, in addition to immunomodulating the IL-8. Therefore, our data proved relevant to introduce a promising therapeutic alternative to toxoplasmosis.

摘要

生物源银纳米颗粒（AgNp-Bio）的显著特点包括低毒性（取决于尺寸和浓度）以及高效的抗寄生虫活性。因此，本研究的目的是评估AgNp-Bio对感染RH株刚地弓形虫的HeLa细胞的作用。首先，我们进行了细胞活力测试并对AgNp-Bio进行了表征，以便用AgNp-Bio或传统药物处理HeLa细胞并使其感染刚地弓形虫。随后，我们测定了标准细胞因子Th1/Th2的水平以及一氧化氮（NO）和活性氧（ROS）的含量。结果表明，AgNp-Bio的平均直径为69纳米，并具有剂量依赖性毒性。此外，3和6μM的浓度显著降低了黏附、感染和细胞内增殖。我们还发现白细胞介素-8（IL-8）和炎症介质的产生减少。因此，纳米颗粒除了对IL-8进行免疫调节外，还降低了ROS和NO的黏附、感染和增殖。因此，我们的数据证明引入一种有前景的弓形虫病治疗替代方法具有重要意义。