College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China.
National Education Examinations Authority, Beijing 100084, PR China.
Life Sci. 2020 Apr 1;246:117432. doi: 10.1016/j.lfs.2020.117432. Epub 2020 Feb 13.
Our previous studies have shown that prenatal cold stress leads to placental inflammatory response and induces anxiety-like behavior reduced in offspring rats. However, the role and mechanisms by which prenatal cold stress affects offspring remain unclear. The aim of this study was to determine the metabolic profiles from the maternal serum and helpful in understanding the role and mechanisms by which prenatal cold stress affects the offspring. In this study, liquid chromatography-mass spectrometry (LC/MS) was used to analyze serum metabolites, and PCA, PLS-DA, and OPLS-DA were performed to analyze changes in metabolites in the maternal serum after cold stress of 3 or 7 days. The results showed that 19 metabolites in the CS (cold stress 7 days)-NS (control) group and 23 metabolites in the CT (cold stress 3 days)-NT (control) group were significantly altered. These metabolites were mainly associated with unsaturated fatty acid synthesis, and arachidonic acid, linoleic acid, and glutamine and glutamate metabolism. The data indicated that prenatal cold stress not only affected the maternal neuroendocrine system, but also affected the immune system, and lipid and amino acid metabolism. These results further supported the findings of our previous studies on the effects of prenatal cold stress on the mother and offspring. A more comprehensive understanding of these data may lead to maternal intervention that can reverse the damage of prenatal stressors.
我们之前的研究表明，产前冷应激会导致胎盘炎症反应，并诱导后代大鼠出现类似焦虑的行为。然而，产前冷应激影响后代的作用和机制尚不清楚。本研究旨在确定母体血清中的代谢谱，以帮助理解产前冷应激影响后代的作用和机制。在这项研究中，我们使用液相色谱-质谱联用技术（LC/MS）分析血清代谢物，并进行 PCA、PLS-DA 和 OPLS-DA 分析，以研究冷应激 3 天或 7 天后母体血清中代谢物的变化。结果表明，CS（冷应激 7 天）-NS（对照）组有 19 种代谢物和 CT（冷应激 3 天）-NT（对照）组有 23 种代谢物发生了显著变化。这些代谢物主要与不饱和脂肪酸合成以及花生四烯酸、亚油酸、谷氨酰胺和谷氨酸代谢有关。这些数据表明，产前冷应激不仅影响母体的神经内分泌系统，还影响免疫系统和脂质及氨基酸代谢。这些结果进一步支持了我们之前关于产前冷应激对母体和后代影响的研究结果。更全面地了解这些数据可能会导致对母体的干预，以逆转产前应激源的损害。
