Department of Clinical Microbiology and Microbial Pathogenesis, University Hospital of Heraklion, Crete, Greece.
University of Crete Medical School, Heraklion, Crete, Greece.
Anaerobe. 2020 Apr;62:102173. doi: 10.1016/j.anaerobe.2020.102173. Epub 2020 Feb 7.
The aim of our study was to determine the antimicrobial susceptibility profiles of 267 Gram-negative clinically significant anaerobes, isolated between October 2016 and October 2019, in a Greek university hospital. The species identification was performed by conventional methods and using the Vitek 2 automated system. Antimicrobial susceptibility testing to determine the MICs was performed by the E-test method. The antimicrobial agents tested were penicillin, ampicillin, amoxicillin-clavulanic acid, piperacillin-tazobactam, cefoxitin, imipenem, meropenem, clindamycin, metronidazole, moxifloxacin, chloramphenicol and tigecycline. The results were interpreted using the CLSI and FDA breakpoints. The majority of the isolates belonged to Bacteroides fragilis group (58.8%), followed by Prevotella spp. (23.2%), Fusobacterium spp. (11.2%) and Veillonella spp. (6.4%). The most prevalent types of infection were skin and soft tissue infections (34.8%), and inta-abdomonal infections (29.6%). Among all isolates tested, the lowest rates of resistance (<5%) were detected to carbapenems, metronidazole, chloramphenicol and tigecycline. Resistance to piperacillin-tazobactam was observed in 5.4%, 24.6%, 3.3% and 17.6%, of B. fragilis, B. fragilis group, Fusobacterium spp. and Veillonella spp. isolates, respectively. Although a high prevalence of resistance to clindamycin, cefoxitin, and moxifloxacin, was detected particularly among members of the B. fragilis group, cefoxitin resistance was low for Prevotella spp. (3.2%), Fusobacterium spp. (3.3%) and Veillonella spp. (0%). Our findings underscore the need for periodic monitoring of antimicrobial resistance in order to guide empirical therapy.
本研究的目的是确定 2016 年 10 月至 2019 年 10 月期间在希腊一所大学医院分离的 267 株临床重要的革兰氏阴性厌氧菌的抗菌药物敏感性谱。通过常规方法和 Vitek 2 自动化系统进行菌种鉴定。采用 E 试验法测定 MIC 以进行抗菌药物敏感性试验。测试的抗菌药物包括青霉素、氨苄西林、阿莫西林-克拉维酸、哌拉西林-他唑巴坦、头孢西丁、亚胺培南、美罗培南、克林霉素、甲硝唑、莫西沙星、氯霉素和替加环素。结果按照 CLSI 和 FDA 折点进行解释。大多数分离株属于脆弱拟杆菌群（58.8%），其次是普雷沃菌属（23.2%）、梭杆菌属（11.2%）和韦荣球菌属（6.4%）。最常见的感染类型是皮肤和软组织感染（34.8%）和腹内感染（29.6%）。在所测试的所有分离株中，对碳青霉烯类、甲硝唑、氯霉素和替加环素的耐药率最低（<5%）。哌拉西林-他唑巴坦的耐药率分别为 5.4%、24.6%、3.3%和 17.6%，在脆弱拟杆菌、脆弱拟杆菌群、梭杆菌属和韦荣球菌属分离株中。虽然克林霉素、头孢西丁和莫西沙星的耐药率在脆弱拟杆菌群中尤其高，但头孢西丁的耐药率在普雷沃菌属（3.2%）、梭杆菌属（3.3%）和韦荣球菌属（0%）中较低。我们的研究结果强调需要定期监测抗菌药物耐药性，以指导经验性治疗。
