Department of Environmental Health Engineering, School of Health, Larestan University of Medical Sciences, Larestan, Iran.
Department of Environmental Health Engineering, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Environ Monit Assess. 2020 Feb 15;192(3):176. doi: 10.1007/s10661-020-8125-5.
Interactive effects of mixtures of pesticides on bacteria are rarely investigated. The aim of this study was to investigate the cumulative, synergistic, and antagonistic effects of four widely used agricultural pesticides including deltamethrin, diazinon, chlorpyrifos, and 2,4-dichlorophenoxyacetic acid (2,4-D) on Pseudomonas, Aeromonas, and Bacillus bacteria. The reduction of alamar blue, as an indicator of bacterial activity, was measured using a spectrophotometer at 600-nm wavelength. Data were analyzed using SPSS 24.0 software. The binary mixtures of pesticides had mainly antagonistic and additive effects, but quadruple mixtures of pesticides had synergistic effects on all of the three bacterial species. In the mixtures of pesticides, increasing the number of compounds leads to more synergistic effects.
目前很少有研究调查农药混合物对细菌的相互作用。本研究旨在调查四种广泛使用的农业农药（包括氯菊酯、二嗪磷、毒死蜱和 2,4-二氯苯氧乙酸（2,4-D））对假单胞菌、气单胞菌和芽孢杆菌的累积、协同和拮抗作用。使用分光光度计在 600nm 波长下测量作为细菌活性指示剂的阿拉马蓝的减少。使用 SPSS 24.0 软件分析数据。农药的二元混合物主要表现出拮抗和相加作用，但四种农药的混合物对所有三种细菌均表现出协同作用。在农药混合物中，化合物数量的增加会导致更多的协同作用。
