College of Public Health, Zhengzhou University, Zhengzhou, 450001, China.
The Key Laboratory of Nanomedicine and Health Inspection of Zhengzhou, Zhengzhou, 450001, China.
Mikrochim Acta. 2020 Feb 15;187(3):171. doi: 10.1007/s00604-019-3914-7.
A method is described for the simultaneous determination of the carcinoembryonic antigen (CEA) and cytokeratin 19 fragment (CYFRA21-1). Two kinds of CdSe/ZnS quantum dot nanobeads (QBs), with emission maxima at 530 nm (green) and 585 nm (yellow), were used as labels, and magnetic beads (MBs) for separation. The MBs were used as substrates to couple CEA and CYFRA21-1 antibody for isolating the proteins. Then, the differently colored QBs were linked to the antibodies against CEA and CYFRA21-1, respectively. Following the formation of the immunocomplex, the intensities of the green and yellow emissions were measured at the same excitation wavelength of 340 nm. The detection limits are 0.1 ng⋅mL for CEA, and of 0.2 ng⋅mL for CYFRA21-1. The recoveries from spiked serum are 92.1 - 118.1% for CEA, and from 90.8% to 115.2% for CYFRA21-1, with the relative standard deviations of 6.3 - 12.3% and 7.1 - 11.8%. The method was successfully applied to the simultaneous determination of the two proteins in human serum sample (n = 45). The results correlated well with those of the chemiluminescent enzyme immunoassay kit. Graphical AbstractSchematic presentation of the fluorescence immunoassay for the simultaneous determination of carcinoembryonic antigen (CEA) and cytokeratin 19 fragment (CYFRA21-1) based on quantum dot nanobeads (QBs) and magnetic beads (MBs) is reported. The intensities of two kinds of CdSe/ZnS QBs, with the emission maxima at 530 nm (green) and 585 nm (yellow) were measured at the same excitation wavelength of 340 nm.
本文介绍了一种同时测定癌胚抗原（CEA）和细胞角蛋白 19 片段（CYFRA21-1）的方法。使用两种发射峰分别位于 530nm（绿色）和 585nm（黄色）的 CdSe/ZnS 量子点纳米珠（QBs）作为标记物，并使用磁性珠（MBs）进行分离。MBs 用作基底，偶联 CEA 和 CYFRA21-1 抗体以分离蛋白质。然后，将不同颜色的 QBs 分别与针对 CEA 和 CYFRA21-1 的抗体连接。形成免疫复合物后，在相同的 340nm 激发波长下测量绿色和黄色发射的强度。CEA 的检测限为 0.1ng·mL，CYFRA21-1 的检测限为 0.2ng·mL。CEA 的回收率为 92.1%至 118.1%，CYFRA21-1 的回收率为 90.8%至 115.2%，相对标准偏差为 6.3%至 12.3%和 7.1%至 11.8%。该方法成功应用于人血清样品中两种蛋白质的同时测定（n=45）。结果与化学发光酶免疫试剂盒的结果相关性良好。
