Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.
Department of Chemical Engineering, University College London , Torrington Place, London WC1E 7JE, United Kingdom.
Anal Chem. 2017 Oct 3;89(19):10556-10564. doi: 10.1021/acs.analchem.7b02777. Epub 2017 Sep 13.
Aflatoxins (AFs) are a class of carcinogens, associated with liver cancers, that exist in foodstuffs. There are extremely low maximum limits of AFs in foodstuffs (0.025-20 μg·kg). Quick and sensitive detection of such low concentration of AFs in foodstuffs is dominated by the efficiency and selectivity of the AF enrichment process, which is extremely challenging although substantial efforts have been made in recent decades. Here we design and synthesize a multilayer nanoarchitecture composed of a broad-spectrum aflatoxin monoclonal antibody shell, chitosan middle layer, and magnetic bead core (denoted AF-mAb/CTS/FeO). The efficiency of AF-mAb/CTS/FeO in extracting AFs has been found to be more than 60 times higher than both conventional immunoaffinity chromatography and solid-phase extraction. Furthermore, the nanocomposite displays excellent selectivity and good reusability as well as outstanding efficiency. When coupled to ultraperformance liquid chromatography-tandem quadrupole mass spectrometry, this new nanoarchitecture enables us to probe six AFs at concentrations as low as 0.003 μg·kg in foodstuffs with free matrix effects, which is nearly 10 times smaller than the regulated maximum tolerated does. It is believed that the new nanoarchitecture will provide an efficient and fast pathway to detect AFs in foodstuffs to protect human being from some critical liver cancers.
黄曲霉毒素（AFs）是一类致癌物质，存在于食品中，与肝癌有关。食品中黄曲霉毒素的最高限量极低（0.025-20μg·kg）。尽管近几十年来已经做出了大量努力，但对食品中如此低浓度的 AFs 的快速、灵敏检测仍然取决于 AF 富集过程的效率和选择性，这极具挑战性。在这里，我们设计并合成了一种由广谱黄曲霉单克隆抗体壳、壳聚糖中间层和磁性珠核组成的多层纳米结构（表示为 AF-mAb/CTS/FeO）。已经发现，AF-mAb/CTS/FeO 提取 AFs 的效率比传统免疫亲和色谱和固相萃取高 60 多倍。此外，该纳米复合材料还表现出优异的选择性、良好的可重复使用性和出色的效率。当与超高效液相色谱-串联四极杆质谱联用时，这种新的纳米结构能够以近乎 10 倍于规定的最大耐受剂量的浓度在食品中检测到六种 AFs，而无需基质效应，其浓度低至 0.003μg·kg。人们相信，这种新的纳米结构将为检测食品中的 AFs 提供一种高效、快速的途径，以保护人类免受一些严重的肝癌的侵害。
