基于表面增强拉曼散射的免疫分析同时检测两种前列腺特异性抗原，用于前列腺癌的准确诊断。

Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer.

机构信息

Department of Bionano Technology, Hanyang University , Ansan 426-791, South Korea.

Department of Laboratory Medicine, Korea University College of Medicine , Seoul 152-854, South Korea.

出版信息

ACS Nano. 2017 May 23;11(5):4926-4933. doi: 10.1021/acsnano.7b01536. Epub 2017 Apr 27.

DOI:10.1021/acsnano.7b01536

PMID:28441008

Abstract

Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.

摘要

准确分析临床血清中的特定生物标志物对于癌症的早期诊断和治疗至关重要。在这里，我们开发了一种基于表面增强拉曼散射（SERS）的免疫分析方法，使用磁性珠和 SERS 纳米标签，用于测定游离至总（f/t）前列腺特异性抗原（PSA）比值，以提高前列腺癌的诊断性能。为了评估该方法的临床适用性，我们对临床样本中在 4.0 和 10.0ng/mL 之间的灰色区域中的两种 PSA 标志物（游离 PSA（f-PSA）和复合 PSA（c-PSA））进行了基于 SERS 的双重 PSA 标志物同时检测的测定。我们的 f/t PSA 比值的测定结果与在大学医院临床实验室安装的电化学发光（ECL）系统测量的结果具有良好的线性相关性。此外，与平行检测相比，在 13 个临床血清样本中同时检测 f-PSA 和 c-PSA 的方法具有更好的精密度。因此，我们基于 SERS 的临床液体制剂中双重 PSA 标志物的同时检测具有在前列腺癌的准确诊断中应用的强大潜力。