Nitobe T, Harata S, Okamoto Y, Nakamura T, Endo M
Department of Orthopaedic Surgery, Hirosaki University School of Medicine, Japan.
Spine (Phila Pa 1976). 1988 Nov;13(11):1332-9. doi: 10.1097/00007632-198811000-00021.
The effects of chymopapain treatment of the canine intervertebral disc were studied in vivo by monitoring proteoglycan in the nucleus pulposus. Analysis of proteoglycan was carried out by Sepharose CL-4B (Pharmacia Fine Chemicals AB, Stockholm, Sweden) chromatography and electrophoresis. The proteoglycan was degraded to glycosaminoglycans within 1 week after chymopapain treatment. Two weeks later, a proteoglycan smaller than the original appeared in the nucleus pulposus. At 8 weeks after injection, the amount of the newly synthesized proteoglycan, similar in molecular weight to the original, had recovered to about half that of the original, although the new proteoglycan fraction was rich in hyaluronic acid. It was concluded that, following chemonucleolysis with chymopapain, the water-binding capacity of the nucleus pulposus recovered, but that the regenerated nucleus pulposus differed biochemically from the original.
通过监测髓核中的蛋白聚糖，在体内研究了木瓜凝乳蛋白酶治疗犬椎间盘的效果。采用Sepharose CL - 4B（瑞典斯德哥尔摩法玛西亚精细化学品公司）色谱法和电泳法对蛋白聚糖进行分析。木瓜凝乳蛋白酶治疗后1周内，蛋白聚糖降解为糖胺聚糖。两周后，髓核中出现了一种比原来更小的蛋白聚糖。注射后8周，新合成的蛋白聚糖分子量与原来相似，但其含量已恢复到原来的约一半，尽管新的蛋白聚糖组分富含透明质酸。得出的结论是，木瓜凝乳蛋白酶化学髓核溶解术后，髓核的水结合能力得以恢复，但再生的髓核在生化性质上与原来的不同。
