Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Dermatology and Andrology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Andrologia. 2020 May;52(4):e13532. doi: 10.1111/and.13532. Epub 2020 Feb 16.
The expression of the main cannabinoid receptors (CBR1 and CBR2) was investigated to evaluate the possible association with the sperm maturation from fertile and infertile individuals. One hundred subjects were classified into fertile (n = 50) and infertile groups (n = 50). Fresh semen samples were collected. Computer-assisted semen analysis and acrosin activity test were done. RNA was extracted from mature and immature sperm pellets. Reverse transcriptase reaction and real-time PCR were done to assess the levels of both CBR1 and CBR2 genes expression in all samples. Mature spermatozoa from both groups showed significantly higher levels of both CBR1 and CBR2 compared with the immature spermatozoa (p < .05). This increment was significantly more important in the fertile group (p < .05). In mature spermatozoa, CBR1 expression was significantly related to variation in sperm morphology, and CBR2 was significantly related to both sperm morphology and linearity index. In conclusion, CBR1 and CBR2 mRNA expression may closely direct the sperm maturation at different steps of the reproductive process.
研究主要大麻素受体（CBR1 和 CBR2）的表达，评估其与生育能力正常和异常个体精子成熟的可能关联。
将 100 例研究对象分为生育能力正常组（n=50）和生育能力异常组（n=50）。采集新鲜精液标本，行计算机辅助精液分析和顶体酶活性检测，提取成熟和未成熟精子的 RNA，行逆转录反应和实时 PCR，评估两组所有样本中 CBR1 和 CBR2 基因表达水平。
两组成熟精子的 CBR1 和 CBR2 表达水平均显著高于未成熟精子（p<.05），其中生育能力正常组的增加更显著（p<.05）。在成熟精子中，CBR1 表达与精子形态变化显著相关，CBR2 表达与精子形态和直线性指数显著相关。
CBR1 和 CBR2 mRNA 表达可能在生殖过程的不同阶段直接指导精子成熟。
