Department of Ophthalmology, 958 Army Hospital, ChongQin, People's Republic of China.
Cutan Ocul Toxicol. 2020 Jun;39(2):106-110. doi: 10.1080/15569527.2020.1730883. Epub 2020 Mar 9.
Lysergic acid diethylamide (LSD) is a powerful hallucinogen with high potential for abuse. There is far less known about its effects on the retina, especially the underlying mechanisms. This study was to investigate the acute toxicity of LSD on the retina of C57 mice and its mechanisms of action. C57 mice were treated with LSD at progressively increasing doses (0.2-1.2 mg/kg) intraperitoneally two times daily for 5 days, mice treated with saline served as negative control. Electroretinography (ERG) was used to test the function of the retina. Toluidine blue staining was used to detect the morphology of the retina. Enzyme-linked immunosorbent assay (ELISA) was used to measure the apoptosis-related factors. Real-time PCR and western blot techniques were used to measure expression changes of genes and proteins, respectively. LSD treatment caused retinal damage, as shown by a decrease in ERG response and the loss of photoreceptor cells. LSD treatment also increased apoptosis through up-regulating the expression of p-JAK1/p-STAT1. Our study indicated that intraperitoneal administration of LSD-induced retinal damage of C57 mice, at least partially through regulating the JAK/STAT pathway.
麦角酸二乙酰胺（LSD）是一种具有高度滥用潜力的强效致幻剂。关于它对视网膜的影响，尤其是其潜在机制，人们知之甚少。本研究旨在探讨 LSD 对 C57 小鼠视网膜的急性毒性及其作用机制。C57 小鼠每日腹腔内分别给予 LSD 递增剂量（0.2-1.2mg/kg）2 次，连续 5 天，生理盐水处理的小鼠作为阴性对照。视网膜电图（ERG）用于测试视网膜功能。甲苯胺蓝染色用于检测视网膜形态。酶联免疫吸附试验（ELISA）用于测量与凋亡相关的因子。实时 PCR 和 Western blot 技术分别用于测量基因和蛋白表达的变化。LSD 处理导致视网膜损伤，表现为 ERG 反应降低和光感受器细胞丢失。LSD 处理还通过上调 p-JAK1/p-STAT1 的表达来增加细胞凋亡。我们的研究表明，腹腔内给予 LSD 可诱导 C57 小鼠视网膜损伤，至少部分通过调节 JAK/STAT 通路。
