Sahmoudi Karima, El Allam Aicha, El Fakihi Sara, Tahoune Hicham, Sadak Abderrahim, El Hafidi Naima, Bourkadi Jamaleddine, El Aouad Rajae, Seghrouchni Fouad
Laboratory of Cellular Immunology, The National Institute of Hygiene, Rabat, Morocco.
Faculty of Sciences, University Mohammed V Agdal, Rabat, Morocco.
J Immunoassay Immunochem. 2020 May 3;41(3):281-296. doi: 10.1080/15321819.2020.1728543. Epub 2020 Feb 15.
Lymphocyte subsets reference ranges are helpful for a precise diagnosis and therapy of various diseases. We attempted in the current study to establish Moroccan lymphocyte reference range and reveal age, gender, ethnicity, income, and instructional levels dependent differences. Lymphocyte subsets percentage and absolute count were determined by 4-color flow cytometry in a population study of 145 adults Moroccan healthy volunteers. Analysis showed significant age-dependent changes. Age was associated with a decrease of naïve CD4+ and CD8+ T cells and an increase of memory CD4+ or CD8+ T cells. Activated CD4+ CD38+ and CD8+ CD38+ T cells, Treg as well as NK cell showed age-dependent alterations. In contrast, B cells remained unchanged. A higher percentage of CD3+ and CD4+ T cells was observed in females while CD8+, B and NK cells count were higher in men. Ethnicity, instructional levels, and personal income seem to not influence lymphocyte subsets reference values. This study provides reference ranges for lymphocyte subsets of healthy Moroccan adults. These results can be used for other North African (Maghrebian) countries considering their geographic, ethnic, economic, and cultural similarities.
淋巴细胞亚群参考范围有助于对各种疾病进行精确诊断和治疗。在本研究中，我们试图建立摩洛哥人的淋巴细胞参考范围，并揭示年龄、性别、种族、收入和教育水平相关的差异。在一项针对145名摩洛哥健康成年志愿者的人群研究中，通过四色流式细胞术测定淋巴细胞亚群百分比和绝对计数。分析显示存在显著的年龄相关变化。年龄与初始CD4+和CD8+T细胞减少以及记忆CD4+或CD8+T细胞增加有关。活化的CD4+CD38+和CD8+CD38+T细胞、调节性T细胞以及自然杀伤细胞均呈现出年龄相关的改变。相比之下，B细胞保持不变。女性中CD3+和CD4+T细胞的百分比更高，而男性中CD8+、B细胞和自然杀伤细胞计数更高。种族、教育水平和个人收入似乎不影响淋巴细胞亚群参考值。本研究提供了摩洛哥健康成年人淋巴细胞亚群的参考范围。鉴于其他北非（马格里布）国家在地理、种族、经济和文化上的相似性，这些结果可用于这些国家。
