Chen Chen, Liu Yan-Mei, Xuan Shu-Xia, Zhou Mei-Fang, Zhou Peng, Cheng Bin, Lin Jin-Duan, Yin Wei-Guo, Li Lin-Hai
Department of Laboratory Medicine, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan, People's Republic of China.
J Inflamm Res. 2021 Dec 31;14:7529-7537. doi: 10.2147/JIR.S343636. eCollection 2021.
To establish and verify the method for detecting the immune phenotype of peripheral blood T lymphocytes by cellular immune chip technology, analyze the immune status, and discuss its clinical diagnostic value of different populations in the Qingyuan area.
First, a cellular immune chip was used to detect the number of T lymphocyte subsets CD3+, CD4+, CD8+, and CD4/CD8, followed by evaluating the accuracy and precision through a comparison with flow cytometry. After passing the performance verification, a large-scale detection was performed by a cellular immune chip in 8389 cases. Immunochip technology detects the expression of T lymphocyte subsets and analyzes the differences in cellular immune function among people with physical examination, inflammation, and cancer, as well as different cancer types and in genders.
The cell immunochip method and flow cytometry method have the same accuracy and precision in detecting specimens, and the former is fast and simple, and is suitable for clinical use; big data analysis is expected to establish a reference range for CD3+, CD4+, and CD8+ T cell counts in Qingyuan. There are statistical differences in CD3+, CD4+, CD8+ T cell counts in physical examination, inflammation and cancer populations; there are also certain differences in CD3+, CD4+, CD8+ T cell counts and CD4/CD8 ratios between different cancer types and different diseases.
The method of cell immunochip technology to detect T lymphocyte subsets is simple and practical, with accurate results and rapid detection. It can be used for immune function monitoring and treatment prognosis evaluation of people with different diseases, and it is worthy of popularization and application in clinical practice.
建立并验证细胞免疫芯片技术检测外周血T淋巴细胞免疫表型的方法，分析免疫状态，探讨其在清远地区不同人群中的临床诊断价值。
首先，使用细胞免疫芯片检测T淋巴细胞亚群CD3+、CD4+、CD8+及CD4/CD8的数量，随后通过与流式细胞术比较评估其准确性和精密度。性能验证通过后，应用细胞免疫芯片对8389例样本进行大规模检测。免疫芯片技术检测T淋巴细胞亚群的表达，分析体检人群、炎症人群和癌症人群以及不同癌症类型和不同性别之间细胞免疫功能的差异。
细胞免疫芯片法与流式细胞术法在检测标本时具有相同的准确性和精密度，且前者快速简便，适用于临床应用；大数据分析有望建立清远地区CD3+、CD4+和CD8+T细胞计数的参考范围。体检人群、炎症人群和癌症人群的CD3+、CD4+、CD8+T细胞计数存在统计学差异；不同癌症类型和不同疾病之间的CD3+、CD4+、CD8+T细胞计数及CD4/CD8比值也存在一定差异。
细胞免疫芯片技术检测T淋巴细胞亚群的方法简单实用，结果准确，检测快速。可用于不同疾病人群的免疫功能监测和治疗预后评估，值得在临床实践中推广应用。
