Department of Orthopaedics, The 8th Medical Center of Chinese PLA General Hospital, Beijing, 100091, China.
CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing, 100190, China.
Macromol Biosci. 2020 Mar;20(3):e1900394. doi: 10.1002/mabi.201900394. Epub 2020 Feb 17.
Many coating materials have been studied to prevent surgical site infections (SSIs). However, antibacterial coating on surfaces show weak adhesion using the traditional titanium (Ti) cage, resulting in low efficacy for preventing SSIs after spinal surgery. Herein, a 3D-printed Ti cage combined with a drug-releasing system is developed for in situ drug release and bacteria killing, leading to prevention of SSIs in vitro and in vivo. First, a 3D-printed Ti cage is designed and prepared by the Electron Beam Melting (EBM) method. Second, polyvinyl alcohol (PVA) containing hydrophilic vancomycin hydrochloride (VH) is scattered across the surface of 3D-printed porous Ti (Ti-VH@PVA) cages. Ti-VH@PVA cages show an efficient drug-releasing profile and excellent bactericidal effect for three common bacteria after more than seven days in vitro. In addition, Ti-VH@PVA cages exhibit reliable inhibition of inflammation associated with Staphylococcus aureus and effective bone regeneration capacity in a rabbit model of SSIs. The results indicate that Ti-VH@PVA cages have potential advantages for preventing SSIs after spinal surgery.
许多涂层材料已被研究用于预防手术部位感染（SSIs）。然而，表面的抗菌涂层在使用传统钛（Ti）笼时表现出较弱的附着力，导致脊柱手术后 SSIs 的预防效果不佳。在此，开发了一种 3D 打印 Ti 笼结合药物释放系统，用于原位药物释放和杀菌，从而预防体外和体内的 SSIs。首先，通过电子束熔化（EBM）方法设计和制备 3D 打印 Ti 笼。其次，将含有亲水性万古霉素盐酸盐（VH）的聚乙烯醇（PVA）散布在 3D 打印多孔 Ti（Ti-VH@PVA）笼的表面上。Ti-VH@PVA 笼在体外超过七天后，对三种常见细菌表现出高效的药物释放特性和优异的杀菌效果。此外，Ti-VH@PVA 笼在金黄色葡萄球菌相关炎症的抑制和 SSIs 兔模型中的有效骨再生能力方面表现出可靠的效果。结果表明，Ti-VH@PVA 笼在预防脊柱手术后 SSIs 方面具有潜在优势。
