School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, India.
School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, India; IMGENEX India Pvt. Ltd., Bhubaneswar, India.
Colloids Surf B Biointerfaces. 2020 May;189:110846. doi: 10.1016/j.colsurfb.2020.110846. Epub 2020 Feb 4.
Rapid increase in the case of bacterial infections is considered as a major public health concern and hence exploration of alternative treatment procedures including development of nanomaterials based therapeutic strategies is receiving much attention. In this aspect, here we investigated the antibacterial efficacy of a simple and potential metal/metal oxide nanocomposite system. Biogenic synthetic protocol was designed for processing of Ag/CaO nanocomposites (NCs). Structural features and morphology of the synthesized nanomaterials were investigated by X-ray diffraction (XRD) and electron microscopy techniques respectively. Optical properties of the nanomaterials were analyzed by UV-vis spectrophotometer. Presence of water and possible impurity molecules on the materials surface was examined by Fourier-transform infrared spectroscopy (FTIR). Effective antibacterial activity of the NCs was observed (within a range of 25-150 μg/mL of NCs) against Staphylococcus aureus (S. aureus) and Methicillin-resistant Staphylococcus aureus (MRSA). The potential anti-biofilm effect of as synthesized NCs was tested against S. aureus. Experimental results suggest that the antibacterial action of the NCs could be due to the induction of reactive oxygen species (ROS). DNA degradation and change in the bacterial cell membrane has further indicated the complete disinfection of the target bacterial system. The cytotoxicity evaluation has confirmed that the formation of NCs has maintained the antibacterial efficacy of Ag NPs but reduced its toxicity towards mammalian cells.
细菌感染病例的迅速增加被认为是一个主要的公共卫生关注点，因此，探索替代治疗方法，包括开发基于纳米材料的治疗策略，正受到越来越多的关注。在这方面，我们研究了一种简单而有潜力的金属/金属氧化物纳米复合材料系统的抗菌功效。我们设计了一种生物合成方案来处理 Ag/CaO 纳米复合材料 (NCs)。通过 X 射线衍射 (XRD) 和电子显微镜技术分别研究了合成纳米材料的结构特征和形态。通过紫外-可见分光光度计分析了纳米材料的光学性质。通过傅里叶变换红外光谱 (FTIR) 检查了材料表面的水和可能的杂质分子的存在。观察到 NCs 对金黄色葡萄球菌 (S. aureus) 和耐甲氧西林金黄色葡萄球菌 (MRSA) 具有有效的抗菌活性（在 25-150μg/mL 的 NCs 范围内）。测试了合成的 NCs 对金黄色葡萄球菌的潜在抗生物膜效应。实验结果表明，NCs 的抗菌作用可能是由于诱导了活性氧 (ROS)。DNA 降解和细菌细胞膜的变化进一步表明，目标细菌系统已被完全消毒。细胞毒性评估证实，NCs 的形成保持了 Ag NPs 的抗菌功效，但降低了其对哺乳动物细胞的毒性。
