Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 171 77 Stockholm, Sweden.
Division of Clinical Microbiology, Karolinska University Hospital, 171 76 Stockholm, Sweden.
ACS Infect Dis. 2021 Aug 13;7(8):2068-2072. doi: 10.1021/acsinfecdis.0c00903. Epub 2021 Mar 29.
Antibacterial activity of silver nanoparticles is often associated with toxicity to the host. We here report that noncytotoxic doses of silver nanoparticles coated with zinc oxide, Ag@ZnO, can stimulate proliferation and migration of human keratinocytes, HaCaT, with increased expression of Ki67 and vinculin at the leading edge of wounds. Interestingly, Ag@ZnO stimulates keratinocytes to produce the antimicrobial peptides hBD2 and RNase7, promoting antibacterial activity against both extracellular and intracellular isolated from wounds. Overall, these results suggest that Ag@ZnO has the potential to significantly improve treatment outcomes in clearing wound infection.
银纳米颗粒的抗菌活性通常与宿主毒性有关。我们在这里报告，用氧化锌包覆的非细胞毒性剂量的银纳米颗粒（Ag@ZnO）可以刺激人角质形成细胞（HaCaT）的增殖和迁移，在伤口的前沿增加 Ki67 和 vinculin 的表达。有趣的是，Ag@ZnO 刺激角质形成细胞产生抗菌肽 hBD2 和 RNase7，增强对伤口中外源和内源的抗菌活性。总的来说，这些结果表明 Ag@ZnO 有可能显著改善清除伤口感染的治疗效果。
