College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China; Swedish Centre for Resource Recovery, University of Borås, 50190 Borås, Sweden.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China.
Bioresour Technol. 2020 May;304:122962. doi: 10.1016/j.biortech.2020.122962. Epub 2020 Feb 6.
The influence of pig manure biochar amendment (PMBA) during the pig manure (PM) and wheat straw (WS) composting was evaluated. Five concentration of PMBA (0%, 2%, 4%, 6% and10%) were applied to explore the bacterial distributions in PM compost by 16SDNA amplicons sequencing. The results showed that the addition of 6% PMBA could significantly enhanced the bacterial community abundance compared with other composts, while control has relative less bacterial population (332 OTU). The visualization of phylogenetic tree and krona demonstrated the distinctive distribution of each composts, suggested that biochar dosages have an influence on bacterial communities' variation during co-composting. Beta-diversity of distance matrix heat-map and principal component analysis confirmed that bacterial communities were considerably correlated with increasing PMBA. Redundancy also confirmed the similarity and discrepancy among all treatments and environmental factors. This work considered as the potential of PMBA as a booster in composting, where T4 has most plentiful bacterial community and diversity.
评估了猪粪生物炭改良（PMBA）在猪粪（PM）和小麦秸秆（WS）堆肥过程中的影响。添加了 5 种浓度的 PMBA（0%、2%、4%、6%和 10%），通过 16S rDNA 扩增子测序来探索 PM 堆肥中的细菌分布。结果表明，与其他堆肥相比，添加 6%的 PMBA 可显著提高细菌群落丰度，而对照处理（即不添加 PMBA）的细菌种群相对较少（332 个 OTU）。系统发育树和 Krona 的可视化显示了每个堆肥的独特分布，表明生物炭用量对共堆肥过程中细菌群落的变化有影响。距离矩阵热图和主成分分析的β多样性证实了细菌群落与 PMBA 的增加密切相关。冗余分析也证实了所有处理和环境因素之间的相似性和差异。这项工作考虑了 PMBA 作为堆肥中的促进剂的潜力，其中 T4 具有最丰富的细菌群落和多样性。
