Frazier A Nathan, Willis William, Robbe Heather, Ortiz Anna, Koziel Jacek A
United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Bushland, TX 79012, USA.
Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA.
Microorganisms. 2025 Mar 27;13(4):758. doi: 10.3390/microorganisms13040758.
Biochar has significant potential for livestock microbiomes and crop agriculture regarding greenhouse gas emissions reduction. Therefore, a pilot study was designed to investigate the effect of biochar application on the surface of swine manure from an open lagoon and the associated microbial communities. Samples were collected from four different treatment groups: control ( = 4), coarse biochar ( = 4), fine biochar ( = 4), and ultra-fine biochar ( = 4). Additionally, aged manure in bulk was collected ( = 4) to assess alterations from the control group. The method of 16S rRNA amplicon sequencing along with microbial analyses was performed. Diversity was significantly different between aged manure in bulk samples and all treatment groups (Kruskal-Wallis; < 0.05). Additionally, distinct community compositions were seen using both weighted and unweighted UniFrac distance matrices (PERMANOVA; < 0.01). Differential abundance analysis revealed four distinct features within all treatment groups that were enriched (q < 0.001): spp., , , and an uncultured Gammaproteobacteria species. Similarly, spp. and (q-value < 0.001) were significantly depleted by all the treatments. Stochastic and functional analyses revealed that biochar treatments were not deterministically altering assembly patterns, and functional redundancy was evident regardless of compositional shifts.
生物炭在减少温室气体排放方面对家畜微生物群和作物农业具有巨大潜力。因此，设计了一项试点研究，以调查生物炭施用于开放式泻湖猪粪表面及其相关微生物群落的影响。从四个不同处理组采集样本：对照组（n = 4）、粗生物炭组（n = 4）、细生物炭组（n = 4）和超细生物炭组（n = 4）。此外，收集了大量陈化粪便（n = 4）以评估与对照组的差异。采用16S rRNA扩增子测序方法并进行微生物分析。大量陈化粪便样本与所有处理组之间的多样性存在显著差异（Kruskal-Wallis检验；P < 0.05）。此外，使用加权和未加权的UniFrac距离矩阵均观察到不同的群落组成（PERMANOVA检验；P < 0.01）。差异丰度分析显示，所有处理组中有四个不同的特征丰度增加（q < 0.001）：芽孢杆菌属、鞘氨醇单胞菌属、假单胞菌属和一种未培养的γ-变形菌纲物种。同样，所有处理均使鞘氨醇单胞菌属和假单胞菌属（q值 < 0.001）显著减少。随机和功能分析表明，生物炭处理并未确定性地改变组装模式，并且无论组成如何变化，功能冗余都很明显。
