College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China.
Sci Total Environ. 2022 Mar 25;814:152688. doi: 10.1016/j.scitotenv.2021.152688. Epub 2021 Dec 30.
In present study, the taxonomic variation of antibiotic resistant bacteria (ARB) in pig manure (PM) composting with coconut shell biochar (CSB) and bamboo biochar (BB) addition was investigated. The experiment was divided into three treatments: T1 (as control or without biochar amendment), T2 was added 10% coconut shell biochar and T3 supplemented with 10% bamboo biochar. The initial feed stock were properly homogenized using a mechanical crusher. PM and wheat straw (WS) were mixed in a 5: 1 dry weight ratio to adjust the initial carbon/nitrogen ratio 25:1, bulk density to ~0.5 (kg/L) and ~60% moisture content, respectively. This experiment was lasted for 42 days. The results indicated the bacterial communities in the three treatments were more different in terms of relative abundance and diversity of dominant bacteria. The control group had the highest abundance of Kingdome bacteria. The changes in ARB was noticed by variation in the relative abundances of Actinobacteria, Proteobacteria, Firmicutes and Bacteroidota. At the end of composting (on day 42), the total RAs of ARB at the class, order, and family levels were considerably reduced in T2 and T3 by ~35.78-38.75%, 36.42-40.63% and 45.82-47.70%, respectively. But in T1 was decreased by 6.16-8.62%, 7.93-8.72% and 8.70-10.15%, as compared with the day 0 sample. However, the CSB was much more effective to reduce 55 to 60% of ARB than T3 or BB applied treatment has 40 to 42% ARB reduction, while control has certainly very less RAa of ARB reduction. Finally, the biochar amendment was significant approach to mitigate the total ARB abundance in compost and it's further used for organic farming purposes.
在本研究中，研究了添加椰子壳生物炭（CSB）和竹生物炭（BB）的猪粪（PM）堆肥中抗生素耐药细菌（ARB）的分类变化。实验分为三个处理：T1（作为对照或不添加生物炭），T2 添加 10%椰子壳生物炭，T3 添加 10%竹生物炭。初始饲料原料用机械破碎机适当混合。PM 和小麦秸秆（WS）以 5:1 的干重比混合，分别调整初始碳/氮比为 25:1、堆积密度为0.5（kg/L）和60%水分含量。该实验持续了 42 天。结果表明，三个处理组的细菌群落在优势细菌的相对丰度和多样性方面差异更大。对照组的 Kingdom 细菌丰度最高。通过 Actinobacteria、Proteobacteria、Firmicutes 和 Bacteroidota 的相对丰度变化，注意到 ARB 的变化。在堆肥结束时（第 42 天），T2 和 T3 中 ARB 的总 RA 在纲、目和科水平上分别减少了约 35.78-38.75%、36.42-40.63%和 45.82-47.70%，但在 T1 中减少了 6.16-8.62%、7.93-8.72%和 8.70-10.15%，与第 0 天样品相比。然而，与 T3 或 BB 应用处理相比，CSB 更有效地减少 55%至 60%的 ARB，而控制处理则减少了 40%至 42%的 ARB，同时控制处理的 ARB 减少率肯定很低。最后，生物炭的添加是减少堆肥中总 ARB 丰度的有效方法，可进一步用于有机农业目的。
