Department of Clinical Immunology, PLA Specialized Research Institute of Rheumatology & Immunology, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Road, Xi'an 710032, Shaanxi, China; Institute of Rheumatology, Tangdu Hospital, Fourth Military Medical University, No. 1 Xinsi Road, Xi'an 710038, Shaanxi, China.
Department of Clinical Immunology, PLA Specialized Research Institute of Rheumatology & Immunology, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Road, Xi'an 710032, Shaanxi, China.
Int Immunopharmacol. 2020 Apr;81:106282. doi: 10.1016/j.intimp.2020.106282. Epub 2020 Feb 14.
To ascertain the number and percentage of angiogenic T (Tang) cell subsets by flow cytometry in systemic sclerosis (SSc), and their relation with specific clinical features. Thirty SSc patients and 15 healthy controls (HCs) were included. Luminex was performed to analyze the levels of interleukin (IL)-17A, vascular endothelial growth factor (VEGF), tumor necrosis factor-α, and vascular cell adhesion molecule (VCAM). The ratio of circulating CD3 + CD31 + CXCR4 + T (CD3 + Tang) cells and CD8+ CD31 + CXCR4 + T (CD8+ Tang) cells in SSc patients was enlarger than in HCs, while CD4 + CD31 + CXCR4 + T cells (CD4 + Tang) exhibited no difference between SSc patients and HCs. The number and percentage of Tang cells were higher in SSc patients with pulmonary artery hypertension (PAH) than in non-PAH SSc patients and HCs. The ratios of Tang cell subsets in nucleolar pattern-positive SSc patients were markedly raised as compared with their negative ones and HCs. Additionally, the percentage of circulating CD3 + Tang cells was positively associated with VEGF serum levels in SSc patients. Meanwhile, the rate of CD8+ tang cells might have been emphatically corresponded to VEGF and VCAM serum levels in SSc patients. These results imply that the increase in Tang cells in peripheral blood are associated with immunoregulatory disturbances in SSc patients.
为了通过流式细胞术确定系统性硬化症 (SSc) 患者中血管生成 T (Tang) 细胞亚群的数量和百分比，以及它们与特定临床特征的关系。共纳入 30 名 SSc 患者和 15 名健康对照者 (HCs)。采用 Luminex 技术分析白细胞介素 (IL)-17A、血管内皮生长因子 (VEGF)、肿瘤坏死因子-α 和血管细胞黏附分子 (VCAM) 的水平。与 HCs 相比，SSc 患者外周血中循环 CD3+CD31+CXCR4+T (CD3+Tang) 细胞和 CD8+CD31+CXCR4+T (CD8+Tang) 细胞的比例更大，而 CD4+CD31+CXCR4+T 细胞 (CD4+Tang) 在 SSc 患者和 HCs 之间无差异。与非肺动脉高压 (PAH) SSc 患者和 HCs 相比，肺动脉高压 (PAH) SSc 患者的 Tang 细胞数量和百分比更高。核仁模式阳性 SSc 患者的 Tang 细胞亚群比例明显高于阴性患者和 HCs。此外，SSc 患者外周血中循环 CD3+Tang 细胞的百分比与 VEGF 血清水平呈正相关。同时，CD8+Tang 细胞的比率可能与 SSc 患者的 VEGF 和 VCAM 血清水平呈显著对应关系。这些结果表明，外周血中 Tang 细胞的增加与 SSc 患者的免疫调节紊乱有关。
