Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Anal Chem. 2020 Mar 3;92(5):3787-3794. doi: 10.1021/acs.analchem.9b05162. Epub 2020 Feb 21.
Aberrant brown adipose tissue (BAT) metabolism is linked to obesity as well as other metabolic disorders. However, the paucity of imaging tools limits the study of in vivo BAT metabolism in animal models. The current work evaluated a heptamethine dye (CyHF-8) in living mice as a dual-modality BAT-avid molecular probe for two imaging approaches, including near-infrared fluorescence imaging (NIRF) and photoacoustic imaging (PAI). CyHF-8 exhibited favorable spectral properties in the near-infrared window (786/787/805 nm) and accumulated in the subcellular mitochondria of brown adipocytes. After intravenous injection of CyHF-8, NIRF and PAI were both capable of noninvasively detecting interscapular BAT at early time points in living mice. Quantitative analysis of NIRF and PAI images showed that CyHF-8 signals respond to dynamic BAT changes in mice stimulated by norepinephrine (NE) and in diabetic mice induced by streptozotocin (STZ). In summary, dual-modality NIRF/PAI probe CyHF-8 can be used for both NIRF and PAI to noninvasively assess BAT metabolism in living animals.
棕色脂肪组织（BAT）代谢异常与肥胖以及其他代谢紊乱有关。然而，成像工具的缺乏限制了动物模型中 BAT 代谢的活体研究。目前的工作评估了一种七甲川花菁染料（CyHF-8）作为两种成像方法的双模式 BAT 亲和分子探针，包括近红外荧光成像（NIRF）和光声成像（PAI）。CyHF-8 在近红外窗口（786/787/805nm）中表现出良好的光谱特性，并在棕色脂肪细胞的亚细胞线粒体中积累。静脉注射 CyHF-8 后，NIRF 和 PAI 都能够在活体小鼠的早期非侵入性地检测肩胛间 BAT。NIRF 和 PAI 图像的定量分析表明，CyHF-8 信号对去甲肾上腺素（NE）刺激的小鼠和链脲佐菌素（STZ）诱导的糖尿病小鼠的动态 BAT 变化有反应。总之，双模式 NIRF/PAI 探针 CyHF-8 可用于 NIRF 和 PAI，以非侵入性地评估活体动物的 BAT 代谢。
