Jiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu Province, People's Republic of China.
BMC Immunol. 2020 Feb 17;21(1):7. doi: 10.1186/s12865-020-0336-6.
Clonorchis sinensis infection could trigger strong immune responses in mice and humans. However, whether the C.sinensis infection has an impact on arthritis is unknown. Here we investigated the effect of C.sinensis infection on type II collagen-induced arthritis in BALB/c mice.
The mice were firstly infected with 45 C.sinensis metacercariae by oral gavage. Four weeks later, arthritis in mice was induced by type II collagen. Joint inflammation with severe redness and swelling in hind paws was observed in type II collagen-induced arthritis (CIA) mice. Besides, the physical activity was significantly reduced, but the respiratory exchange ratio was increased in CIA mice. Compared with CIA mice, C.sinensis infection could increase the severity of arthritis in CIA mice, based on the results of disease score and pathological changes. Compared to CIA mice, increased neutrophils and Ly6C monocytes, decreased B cells and CD4T cells, were found in C.sinensis infected CIA mice. Besides these, C.sinensis infected mice also displayed significantly higher levels of serum IL-4 and IL-17 than those in CIA mice.
Taken together, our data suggest that C.sinensis infection have a bad effect on arthritis, and could induce the abnormality of the immune response in mice with CIA.
华支睾吸虫感染可在小鼠和人类中引发强烈的免疫反应。然而，华支睾吸虫感染是否对关节炎有影响尚不清楚。在这里，我们研究了华支睾吸虫感染对 BALB/c 小鼠 II 型胶原诱导性关节炎的影响。
首先通过口服灌胃将 45 个华支睾吸虫囊蚴感染给小鼠。四周后，通过 II 型胶原诱导小鼠发生关节炎。在 II 型胶原诱导性关节炎（CIA）小鼠中，观察到后足出现严重红肿和肿胀的关节炎症。此外，CIA 小鼠的身体活动明显减少，但呼吸交换率增加。与 CIA 小鼠相比，华支睾吸虫感染可增加 CIA 小鼠关节炎的严重程度，这基于疾病评分和组织病理学变化的结果。与 CIA 小鼠相比，在感染了华支睾吸虫的 CIA 小鼠中发现了更多的中性粒细胞和 Ly6C 单核细胞，更少的 B 细胞和 CD4T 细胞。此外，与 CIA 小鼠相比，感染了华支睾吸虫的小鼠血清中 IL-4 和 IL-17 的水平也显著升高。
综上所述，我们的数据表明，华支睾吸虫感染对关节炎有不良影响，并可诱导 CIA 小鼠免疫反应异常。
