Human Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Tarbiat Modares University, Faculty of biological sciences, Tehran, Iran.
Genomics. 2020 May;112(3):2615-2622. doi: 10.1016/j.ygeno.2020.02.012. Epub 2020 Feb 14.
Lung cancer is a leading cause of cancer-related death in the world. Therefore, identifying the genes and molecular pathways involved in lung development and tumorigenesis can help us improve the therapeutic strategies of lung cancer. Accumulating evidence confirms that long noncoding RNAs, as a novel layer of regulatory RNA molecules, play an important role in various aspects of the cells. Here, using available high throughput gene expression data, we identified an lncRNA (HSPC324) with high expression level in lung tissue that is distinctly expressed in lung tumor tissues relative to normal. Using GO enrichment and KEGG pathway analyses, we further analyzed the functions and pathways involving the HSPC324-correlated genes. Ectopic expression of lncRNA HSPC324 significantly inhibited proliferation, cell cycle and migration; on the other hand, increased apoptosis and ROS production in lung adenocarcinoma cells. Overall, this study introduces HSPC324 as a new player in the development of lung cancer.
肺癌是全球癌症相关死亡的主要原因。因此，鉴定参与肺发育和肿瘤发生的基因和分子途径可以帮助我们改进肺癌的治疗策略。越来越多的证据证实，长非编码 RNA 作为一种新型的调节 RNA 分子，在细胞的各个方面发挥着重要作用。在这里，我们使用现有的高通量基因表达数据，鉴定出一种在肺组织中高表达的 lncRNA（HSPC324），其在肺肿瘤组织中的表达明显高于正常组织。通过 GO 富集和 KEGG 途径分析，我们进一步分析了与 HSPC324 相关基因相关的功能和途径。lncRNA HSPC324 的异位表达显著抑制了肺腺癌细胞的增殖、细胞周期和迁移；另一方面，增加了细胞凋亡和 ROS 的产生。总的来说，这项研究介绍了 HSPC324 作为肺癌发展的一个新的参与者。
