State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Xueyuan West Road, Lucheng District, Wenzhou 325027, China.
Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Xinsan Road, Longwan District, Wenzhou 325001, China.
ACS Appl Mater Interfaces. 2020 Mar 18;12(11):13256-13264. doi: 10.1021/acsami.9b20794. Epub 2020 Feb 28.
Polysaccharides have recently attracted increasing attention in the construction of hydrogel devices for biomedical applications. However, polysaccharide-based hydrogels are not suitable for most preclinical applications because of their limited mechanical properties and poor tunability. In this study, we employed a simple and eco-friendly approach to producing a macroporous polysaccharide hydrogel composed of salecan and κ-carrageenan without the use of toxic chemicals. We evaluated the physicochemical properties of the obtained salecan/κ-carrageenan hydrogel and found that its viscoelasticity, morphology, swelling, and thermal stability could be simply controlled by changing the polysaccharide dose in the pre-gel solution. The co-incubation of the fabricated hydrogel with mouse fibroblast cells demonstrated that the hydrogel can support cell adhesion, migration, and growth. Moreover, the hydrogel exhibited good biocompatibility in vivo. Overall, the findings provide a new strategy for the fabrication and optimization of polysaccharide-based hydrogel scaffolds for application in tissue engineering.
多糖在用于生物医学应用的水凝胶器件的构建中最近引起了越来越多的关注。然而，由于其机械性能有限和可调性差，基于多糖的水凝胶并不适合大多数临床前应用。在这项研究中，我们采用了一种简单且环保的方法来生产由 salecan 和 κ-卡拉胶组成的具有大孔结构的多糖水凝胶，而无需使用有毒化学物质。我们评估了所获得的 salecan/κ-卡拉胶水凝胶的物理化学性质，发现通过改变预凝胶溶液中的多糖剂量，可以简单地控制其粘弹性、形态、溶胀性和热稳定性。将制备的水凝胶与小鼠成纤维细胞共孵育表明，该水凝胶可以支持细胞黏附、迁移和生长。此外，该水凝胶在体内具有良好的生物相容性。总体而言，这些发现为制造和优化用于组织工程的多糖基水凝胶支架提供了一种新策略。
