Polymer Chemistry & Biomaterials Group-Centre of Macromolecular Chemistry (CMaC)-Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281, S4-Bis, 9000 Ghent, Belgium; Brussels Photonics (B-PHOT)-Department of Applied Physics and Photonics, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
Tissue Engineering and Biomaterials-Department of Basic Medical Sciences, Ghent University, De Pintelaan 185, 6B3, 9000 Ghent, Belgium.
Carbohydr Polym. 2018 Jun 1;189:1-9. doi: 10.1016/j.carbpol.2018.02.002. Epub 2018 Feb 6.
The current paper focuses on the functionalization of κ-carrageenan and gelatin as extracellular matrix polysaccharide and protein mimic respectively to produce hydrogel films for adipose tissue engineering. More specifically, κ-carrageenan as well as gelatin have been functionalized with methacrylate and methacrylamide moieties respectively to enable subsequent UV-induced crosslinking in the presence of a photo-initiator. The gel fraction, the mass swelling ratio and the mechanical properties of both the one-component hydrogels and the protein/polysaccharide blends have been evaluated. The mechanical and swelling properties of the blends could be tuned by varying the hydrogel composition as well as the crosslinking method applied. The in vitro biocompatibility assays indicated a significantly higher cell viability of adipose tissue-derived mesenchymal stem cells seeded onto the blends as compared to the one-component hydrogels. The results show that the blends of gelatin and κ-carrageenan clearly outperform the one-component hydrogels in terms of adipose tissue engineering potential.
本文重点研究了κ-卡拉胶和明胶的功能化，分别将其作为细胞外基质多糖和蛋白质模拟物，以制备用于脂肪组织工程的水凝胶膜。更具体地说，κ-卡拉胶和明胶分别用甲基丙烯酰基和丙烯酰胺基进行了功能化，以便在光引发剂存在下进行随后的 UV 诱导交联。评估了两种单组分水凝胶和蛋白质/多糖共混物的凝胶分数、质量溶胀比和机械性能。通过改变水凝胶组成以及应用的交联方法，可以调节共混物的机械和溶胀性能。体外细胞相容性试验表明，与单组分水凝胶相比，接种到共混物上的脂肪组织来源间充质干细胞的细胞活力明显更高。结果表明，在脂肪组织工程潜力方面，明胶和κ-卡拉胶的共混物明显优于单组分水凝胶。
