State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Xueyuan West Road, Lucheng District, Wenzhou 325027, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Xinsan Road, Lonwan District, Wenzhou 325001, China.
Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Xinsan Road, Lonwan District, Wenzhou 325001, China.
Carbohydr Polym. 2020 Jun 1;237:116160. doi: 10.1016/j.carbpol.2020.116160. Epub 2020 Mar 16.
Polysaccharides derived from microorganisms have received considerable attention in designing hydrogel materials. However, most microbial polysaccharide-constructed hydrogels evaluated in preclinical trials are not favorable candidates for biomedical applications owing to concerns regarding poor mechanical strength and complicated fabrication process. Herein, we describe a new polysaccharide hydrogel scaffold containing salecan together with gellan gum network as the polymeric matrix. Properly controlling the physical and chemical properties including swelling, water release, thermal stability, viscoelasticity and morphology of the resulting gel are easily achieved by simply changing the salecan/gellan gum ratios. Notably, these salecan/gellan gum scaffolds friendly support cell survival and proliferation. More significantly, we have systematically evaluated these developed hydrogels for the biocompatible experiments in vitro and in vivo and results indicated the products are non-toxic. Taken together, such hydrogels derived from microbial polysaccharides and readily synthesized through a one-step mixing protocol have translational potentials in the clinic serving as cell devices for tissue engineering.
微生物来源的多糖在水凝胶材料的设计中受到了广泛关注。然而，由于对机械强度差和复杂的制造工艺的担忧，大多数在临床前试验中评估的微生物多糖构建的水凝胶并不是生物医学应用的理想候选物。在这里，我们描述了一种新的多糖水凝胶支架，其中含有 salecan 与结冷胶网络作为聚合物基质。通过简单地改变 salecan/gellan 胶的比例，很容易控制所得凝胶的物理和化学性质，包括溶胀、水释放、热稳定性、粘弹性和形态。值得注意的是，这些 salecan/gellan 支架有利于细胞的存活和增殖。更重要的是，我们已经系统地评估了这些开发的水凝胶的体外和体内的生物相容性实验，结果表明这些产品是无毒的。总之，这种由微生物多糖衍生而来的水凝胶可以通过一步混合的方法轻松合成，具有转化为组织工程中细胞器件的临床应用潜力。
