El Khoury Marianne, Haykal Tony, Hodroj Mohammad H, Najem Sonia Abou, Sarkis Rita, Taleb Robin I, Rizk Sandra
Department of Natural Sciences, Lebanese American University, Byblos 1401, Lebanon.
Laboratory of Regenerative Hematopoiesis, Swiss Institute for Experimental Cancer Research (ISREC) & Institute of Bioengineering (IBI), School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Cancers (Basel). 2020 Feb 13;12(2):435. doi: 10.3390/cancers12020435.
Webb & Berthel. is a plant from the Malvaceae family that has long been included in the human diet due to its various curative effects. Many plant leaf extracts from the various species of genus have been reported to possess anti-cancer properties, however, studies on Webb & Berthel. leaves have documented anti-inflammatory and anti-oxidant effects with no emphasis on their possible anti-cancer potential. The present study explores the anti-cancer properties of Webb & Berthel. leaf extract on acute myeloid leukemia (AML) cell lines in vitro and deciphers the underlying molecular mechanism. Treatment of AML cell lines with methanolic leaf extract showed a dose- and time-dependent inhibition of proliferation and a dose-dependent increase in apoptotic hallmarks such as an increase in phosphatidylserine on the outer membrane leaflet and membrane leakage in addition to DNA fragmentation. The pro-apoptotic effect was induced by reactive oxygen species (ROS) as well as an upregulation of cleaved poly(ADP-ribose) polymerase (PARP), increase in Bax/Bcl-2 ratio, andrelease of cytochrome-c from the mitochondria. Major compounds of the extract included methyl linolenate, phytol, γ-sitosterol, and stigmasterol as revealed by gas chromatography coupled with mass spectrometry, and amino acids, amino acid derivatives, tiliroside, 13-hydroxyperoxyoctadecadienoic, and quercitrin as detected by liquid chromatography coupled to mass spectrometry.
韦伯氏棉葵是锦葵科的一种植物，由于其多种治疗功效，长期以来一直被纳入人类饮食。据报道，该属不同物种的许多植物叶提取物具有抗癌特性，然而，对韦伯氏棉葵叶的研究记录了其抗炎和抗氧化作用，并未强调其可能的抗癌潜力。本研究探讨了韦伯氏棉葵叶提取物对急性髓系白血病（AML）细胞系的体外抗癌特性，并解读了其潜在的分子机制。用甲醇叶提取物处理AML细胞系显示出增殖的剂量和时间依赖性抑制，以及凋亡特征的剂量依赖性增加，如外膜小叶上磷脂酰丝氨酸的增加、膜泄漏以及DNA片段化。促凋亡作用是由活性氧（ROS）诱导的，同时还伴随着裂解的聚（ADP-核糖）聚合酶（PARP）的上调、Bax/Bcl-2比值的增加以及细胞色素c从线粒体的释放。气相色谱-质谱联用显示提取物的主要化合物包括亚麻酸甲酯、叶绿醇、γ-谷甾醇和豆甾醇，液相色谱-质谱联用检测到氨基酸、氨基酸衍生物、椴树苷、13-羟基过氧化十八碳二烯酸和槲皮苷。
