Lee Seo Yeon, Shrestha Sita, Shrestha Bishnu Kumar, Park Chan Hee, Kim Cheol Sang
Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, Korea.
Division of Mechanical Design Engineering, Jeonbuk National University, Jeonju 561-756, Korea.
Polymers (Basel). 2020 Feb 13;12(2):439. doi: 10.3390/polym12020439.
Herein, we describe precisely a covalent modification of pure magnesium (Mg) surface and its application to induce in vitro osteogenic differentiation. The new concept of a chemical bonding method is proposed for developing stable chemical bonds on the Mg surface through the serial assembly of bioactive additives that include ascorbic acid (AA) and bovine serum albumin (BSA). We studied both the physicochemical and electrochemical properties using scanning electron microscopy and other techniques to confirm how the covalent bonding of BSA on Mg can, after coating, significantly enhance the chemical stability of the substrate. The modified Mg-OH-AA-BSA exhibits better anti-corrosion behavior with high corrosion potential (E = -0.96 V) and low corrosion current density (I = 0.2 µA cm) as compared to the pure Mg (E = -1.46 V, I = 10.42 µA cm). The outer layer of BSA on Mg protects the fast degradation rate of Mg, which is the consequence of the strong chemicals bonds between amine groups on BSA with carboxylic groups on AA as the possible mechanism of peptide bonds. Collectively, the results suggest that the surface-modified Mg provides a strong bio-interface, and enhances the proliferation and differentiation of pre-osteoblast (MC3T3-E1) cells through a protein-lipid interaction. We therefore conclude that the technique we describe provides a cost-effective and scalable way to generate chemically stable Mg surface that inherits a biological advantage in orthopedic and dental implants in clinical applications.
在此，我们精确描述了纯镁（Mg）表面的共价修饰及其在诱导体外成骨分化中的应用。提出了一种化学键合方法的新概念，通过包括抗坏血酸（AA）和牛血清白蛋白（BSA）在内的生物活性添加剂的系列组装，在镁表面形成稳定的化学键。我们使用扫描电子显微镜和其他技术研究了物理化学和电化学性质，以确认涂覆后 BSA 在 Mg 上的共价键合如何显著提高基底的化学稳定性。与纯镁（E = -1.46 V，I = 10.42 μA/cm）相比，改性后的 Mg-OH-AA-BSA 具有更好的抗腐蚀性能，具有高腐蚀电位（E = -0.96 V）和低腐蚀电流密度（I = 0.2 μA/cm）。Mg 上的 BSA 外层保护了 Mg 的快速降解速率，这是 BSA 上的胺基与 AA 上的羧基之间形成强化学键的结果，可能是肽键形成的机制。总体而言，结果表明表面改性的 Mg 提供了一个强大的生物界面，并通过蛋白质-脂质相互作用增强了前成骨细胞（MC3T3-E1）的增殖和分化。因此，我们得出结论，我们所描述的技术提供了一种经济高效且可扩展的方法，以生成在临床应用中在骨科和牙科植入物方面具有生物学优势的化学稳定的镁表面。
