精子在动物克隆中的作用的研究进展。
Insights into the roles of sperm in animal cloning.
机构信息
Laboratory Animal Center, Xi'an Jiaotong University Health Science Center, No.76, Yanta West Road, Xi'an, 710061, Shaanxi, China.
Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.
出版信息
Stem Cell Res Ther. 2020 Feb 18;11(1):65. doi: 10.1186/s13287-020-01599-6.
Somatic cell nuclear transfer (SCNT) has shown a wide application in the generation of transgenic animals, protection of endangered animals, and therapeutic cloning. However, the efficiency of SCNT remains very low due to some poorly characterized key factors. Compared with fertilized embryos, somatic donor cells lack some important components of sperm, such as sperm small noncoding RNA (sncRNA) and proteins. Loss of these factors is considered an important reason for the abnormal development of SCNT embryo. This study focused on recent advances of SCNT and the roles of sperm in development. Sperm-derived factors play an important role in nucleus reprogramming and cytoskeleton remodeling during SCNT embryo development. Hence, considering the role of sperm may provide a new strategy for improving cloning efficiency.
体细胞核移植（Somatic cell nuclear transfer，SCNT）在转基因动物的产生、濒危动物的保护和治疗性克隆方面显示出广泛的应用。然而，由于一些特征不明确的关键因素，SCNT 的效率仍然很低。与受精卵相比，体细胞核供体细胞缺乏精子的一些重要成分，如精子小非编码 RNA（sncRNA）和蛋白质。这些因素的缺失被认为是 SCNT 胚胎发育异常的一个重要原因。本研究重点介绍了 SCNT 的最新进展和精子在胚胎发育中的作用。精子衍生因子在 SCNT 胚胎发育过程中核重编程和细胞骨架重构中发挥重要作用。因此，考虑精子的作用可能为提高克隆效率提供新的策略。
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