兔体细胞核移植。

Somatic Cell Nuclear Transfer in Rabbits.

机构信息

Laboratory Animal Center, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.

Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi'an, Shaanxi, China.

出版信息

Methods Mol Biol. 2023;2647:169-181. doi: 10.1007/978-1-0716-3064-8_8.

DOI:10.1007/978-1-0716-3064-8_8

PMID:37041334

Abstract

Somatic cell nuclear transfer (SCNT) is a technology that enables differentiated somatic cells to acquire a totipotent state, thus making it of great value in developmental biology, biomedical research, and agricultural applications. Rabbit cloning associated with transgenesis has the potential to improve the applicability of this species for disease modeling, drug testing, and production of human recombinant proteins. In this chapter, we introduce our SCNT protocol for the production of live cloned rabbits.

摘要

体细胞核移植（Somatic Cell Nuclear Transfer，SCNT）是一种能够使分化的体细胞获得全能性的技术，因此在发育生物学、生物医学研究和农业应用中具有重要价值。与转基因技术相关的兔克隆有可能提高该物种在疾病建模、药物测试和生产人重组蛋白方面的适用性。在本章中，我们介绍了用于生产活克隆兔的 SCNT 方案。