Department of Nanoengineering, University of California San Diego, La Jolla, CA 92093, USA; Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur, 303002, India.
Department of Nanoengineering, University of California San Diego, La Jolla, CA 92093, USA.
Talanta. 2020 May 1;211:120757. doi: 10.1016/j.talanta.2020.120757. Epub 2020 Jan 16.
Driving under the influence of cannabis and alcohol represents a major safety concern due to the synergistic or additive effect of these substances of abuse. Hence, rapid road-site testing of these substances is highly desired to reduce risks of fatal accidents. Here we describe a wearable electrochemical sensing device for the simultaneous direct, decentralized, detection of salivary THC and alcohol. The new ring-based sensing platform contains a voltammetric THC sensor and an amperometric alcohol biosensor on the ring cap, along with the wireless electronics embedded within the ring case. Rapid replacement of the disposable sensing-electrode ring cap following each saliva assay is accomplished by aligning spring-loaded pins, mounted on the electronic board (PCB), with the current collectors of the sensing electrodes. The printed dual-analyte sensor ring cover is based on a MWCNT/carbon electrode for the THC detection along with a Prussian-blue transducer, coated with alcohol oxidase/chitosan reagent layer, for the biosensing of alcohol. THC and alcohol can thus be detected simultaneously in the same diluted saliva sample within 3 min, with no cross talk and no interferences from the saliva matrix. The new wearable ring sensor platform should enable law enforcement personnel to screen drivers in a single traffic stop and offers considerable promise for addressing growing concerns of drug-impaired driving.
大麻和酒精的影响下驾驶是一个主要的安全问题，因为这些滥用物质具有协同或叠加效应。因此，非常需要在现场快速检测这些物质，以降低致命事故的风险。在这里，我们描述了一种用于同时直接、分散检测唾液中 THC 和酒精的可穿戴电化学传感装置。新的基于环的传感平台在环盖上包含一个伏安 THC 传感器和一个安培酒精生物传感器，以及嵌入在环壳内的无线电子设备。通过对齐安装在电子板 (PCB) 上的带弹簧的销钉与传感电极的集流器，可在每次唾液分析后快速更换一次性传感电极环盖。印刷的双分析物传感器环盖基于 MWCNT/碳电极用于 THC 检测，以及涂有酒精氧化酶/壳聚糖试剂层的普鲁士蓝换能器用于酒精的生物传感。因此，THC 和酒精可以在 3 分钟内同时在相同稀释的唾液样本中检测到，没有串扰，也没有唾液基质的干扰。这种新的可穿戴环传感器平台应该能够使执法人员在一次交通拦截中对驾驶员进行筛查，并为解决日益增长的药物驾驶担忧提供很大的希望。
