Saljoughi Hamidreza, Khakbaz Faeze, Mahani Mohamad
Jiroft University of Medical Sciences, Jiroft, Iran.
Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran.
Photodiagnosis Photodyn Ther. 2020 Jun;30:101687. doi: 10.1016/j.pdpdt.2020.101687. Epub 2020 Feb 15.
Folic acid functionalized carbon quantum dots (FA-CQDs) with ultrahigh quantum yield (50 %) were synthesized by one-pot hydrothermal route using citric acid. The synthesized CQDs were characterized by fluorescence spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS) and X-ray diffraction. The cell viability of about 95 % and 97 % were obtained for MTT assay of the CQDs and FA-CQDs toward MCF-7 cells after 24 h of incubation respectively. The FA-CQDs were successfully applied for targeted imaging of ovarian cancer (type HeLa) and human breast adenocarcinoma (type MCF7) cells using fluorescence microscope.
采用柠檬酸通过一锅水热法合成了具有超高量子产率（50%）的叶酸功能化碳量子点（FA-CQDs）。通过荧光光谱、透射电子显微镜（TEM）、动态光散射（DLS）和X射线衍射对合成的碳量子点进行了表征。分别在孵育24小时后，对CQDs和FA-CQDs进行MTT测定，结果显示它们对MCF-7细胞的细胞活力分别约为95%和97%。利用荧光显微镜，FA-CQDs成功应用于卵巢癌（HeLa型）和人乳腺腺癌（MCF7型）细胞的靶向成像。
