State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China.
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China; State Key Laboratory of Large Yellow Croaker Breeding, Ningde Fufa Fisheries Company Limited, Ningde, 352130, China.
Fish Shellfish Immunol. 2020 Apr;99:462-472. doi: 10.1016/j.fsi.2020.02.024. Epub 2020 Feb 16.
Large yellow croaker (Larimichthys crocea) is one of the most important mariculture fish in China. In the past decades, cryptocaryonosis caused by Cryptocryon irritans has led to huge economic losses, posing great threat to the healthy and sustainable development of L. crocea mariculture industry. As the largest immunologically active mucosal organ in fish, skin provides the first defense line against external pathogens. To better understand the gene expression dynamics, the large yellow croakers were artificially infected with C. irritans and their skin tissues were collected at 0 h, 24 h, 48 h, 72 h and 96 h post infection. The total RNA in the skin tissues were extracted and the transcriptome were sequenced. After sequencing, a total of 1,131, 311, 140 million high quality RNA-seq reads were collected. A set of 215, 473, 968, 1055 differentially expressed genes were identified at 24 h, 48 h, 72 h and 96 h post infection respectively. Further analysis clustered these DEGs into six profiles and 75 hub genes for six profiles were identified. Among these hub genes, 18 immune related genes including TLR5, TOPK, NFKBIZ, MAPK14A were identified post C. irritans infection. Cytokine-cytokine receptor interaction was the only pathway that significantly enriched at four timepoints post infection. This study provides an in-depth understanding of skin transcriptome variance of large yellow croaker after C. irritans infection, which would be helpful for further understanding of the molecular mechanism of L. crocea in response to C. irritans infection.
大黄鱼（Larimichthys crocea）是中国最重要的海水养殖鱼类之一。在过去的几十年中，由 Cryptocryon irritans 引起的隐核虫病导致了巨大的经济损失，对大黄鱼海水养殖业的健康和可持续发展构成了巨大威胁。皮肤作为鱼类最大的免疫活性黏膜器官，为鱼类提供了抵御外来病原体的第一道防线。为了更好地了解基因表达动态，本研究采用人工感染方式将大黄鱼感染隐核虫，并在感染后 0 h、24 h、48 h、72 h 和 96 h 采集皮肤组织，提取总 RNA 进行转录组测序。测序后，共获得了 1131 条、311 条、1400 条和 968 条高质量的 RNA-seq reads。在感染后 24 h、48 h、72 h 和 96 h 分别鉴定到了 215 个、473 个、968 个和 1055 个差异表达基因。进一步分析将这些差异表达基因聚类为六个表达谱，并鉴定到了六个表达谱的 75 个枢纽基因。在这些枢纽基因中，鉴定到了包括 TLR5、TOPK、NFKBIZ 和 MAPK14A 在内的 18 个免疫相关基因在感染隐核虫后表达上调。在四个时间点感染后，细胞因子-细胞因子受体相互作用是唯一显著富集的通路。本研究深入了解了大黄鱼感染隐核虫后皮肤转录组的变化，有助于进一步了解大黄鱼对隐核虫感染的分子机制。
