National and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, 316022, China.
Marine Fishery Institute of Zhejiang Province, Key Lab of Mariculture and Enhancement of Zhejiang Province, Zhoushan, 316100, China.
Fish Shellfish Immunol. 2024 Jan;144:109258. doi: 10.1016/j.fsi.2023.109258. Epub 2023 Nov 30.
Large yellow croaker (Larimichthys crocea) is the most productive marine fish in China. Cryptocaryon irritans is an extremely destructive parasite that causes great economic losses in large yellow croaker aquaculture industry. Therefore, it is very necessary to study the immune response of large yellow croaker in response to C. irritans infection. In this study, the transcriptomic profiles of large yellow croaker were sequenced and analyzed in the brain and head kidney at 72 h after C. irritans infection. Cytokines and chemokines related terms were significantly enriched based on the GO enrichment of down-regulated differentially expressed genes (DEGs) from the head kidney. Meanwhile, cytokine-cytokine receptor interaction was significantly enriched based on the KEGG enrichment of up-regulated DEGs from the brain and down-regulated DEGs from the head kidney, respectively. Moreover, the majority of inflammation-related DEGs were significantly up-regulated in the brain, but distinctly down-regulated in the head kidney. These results showed that the brain and head kidney might play different roles against C. irritans infection, and the inflammatory response of large yellow croaker may be restrained during C. irritans infection. Taken together, the transcriptomic analyses will be helpful to more comprehensively understand the immune mechanism of teleost against C. irritans infection, and provide a theoretical basis for the prevention and treatment of Cryptosporidiosis.
大黄鱼（Larimichthys crocea）是中国产量最高的海洋鱼类。刺激隐核虫是一种极具破坏性的寄生虫，它给大黄鱼养殖业造成了巨大的经济损失。因此，研究大黄鱼对刺激隐核虫感染的免疫反应非常必要。在这项研究中，我们对刺激隐核虫感染后 72 小时的大黄鱼大脑和头肾进行了转录组测序和分析。基于头肾下调差异表达基因（DEGs）的 GO 富集分析，显著富集了与细胞因子和趋化因子相关的术语。同时，基于大脑上调 DEGs 和头肾下调 DEGs 的 KEGG 富集分析，细胞因子-细胞因子受体相互作用显著富集。此外，大多数与炎症相关的 DEGs 在大脑中显著上调，而在头肾中显著下调。这些结果表明，大脑和头肾可能在对抗刺激隐核虫感染方面发挥不同的作用，大黄鱼的炎症反应可能在刺激隐核虫感染期间受到抑制。综上所述，转录组分析将有助于更全面地了解鱼类对抗刺激隐核虫感染的免疫机制，并为隐孢子虫病的防治提供理论依据。
