Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China; Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China.
Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China.
Bioresour Technol. 2020 May;304:123007. doi: 10.1016/j.biortech.2020.123007. Epub 2020 Feb 11.
Studying biohydrogen production from alfalfa is of practical significance to cleaner production and biomass utilization. The performances of biohydrogen production through active/passive saccharification and photo-fermentation were compared. The effects of initial pH, substrate concentration, and cellulase loading on biohydrogen production from alfalfa by photosynthetic bacteria HAU-M1 were presented. It was found that the maximum hydrogen yield of 55.81 mL/g was achieved at initial pH of 6.90, substrate concentration of 31.23 g/mL, and cellulase loading of 0.13 g/g. Hydrogen yield of active saccharification and photo-fermentation was much higher as compare to passive saccharification and photo-fermentation. Initial pH value showed a more significant influence on photosynthetic bacteria in comparison to cellulase in active saccharification and photo-fermentation biohydrogen production. The low yield of propionic acid suggested that it was an efficient photosynthetic hydrogen production. Photo-fermentation hydrogen production from alfalfa provides a novel path for efficient utilization of alfalfa.
研究苜蓿生物制氢对清洁生产和生物质利用具有重要意义。本文对比了活性/ 被动糖化和光发酵生产生物氢气的性能，介绍了初始 pH 值、底物浓度和纤维素酶用量对 HAU-M1 光合细菌发酵苜蓿产氢的影响。结果表明，在初始 pH 值 6.90、底物浓度 31.23 g/mL 和纤维素酶用量 0.13 g/g 条件下，最大产氢量为 55.81 mL/g。与被动糖化和光发酵相比，活性糖化和光发酵的氢气产量要高得多。与活性糖化和光发酵生物制氢相比，初始 pH 值对光合细菌的影响比纤维素酶更为显著。丙酸产量较低表明这是一种高效的光合产氢。从苜蓿中进行光发酵产氢为高效利用苜蓿提供了一条新途径。
