Fisheries Institute, Sichuan Academy of Agricultural Sciences, Chengdu 611731, Sichuan, China.
Sichuan Academy of Agricultural Sciences, Chengdu 610066, Sichuan, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2020 Jun;34:100659. doi: 10.1016/j.cbd.2020.100659. Epub 2020 Feb 10.
Leiocassis longirostris is a common fish variety that is widely cultivated in China, during the breeding process however, it is highly susceptible to bacterial haemorrhagic septicemia, which can cause great economic loss for farmers. To understand the immune responses of L. longirostris to Aeromonas hydrophila infection, Illumina sequencing was employed to identify changes in the mRNA and miRNA in spleen tissue. In this study, a total of 92.16 and 95.61 million (M) high-quality transcriptome reads were generated from the control group (CG) and experimental group (EG) spleen samples, respectively, and 207 up-regulated and 185 down-regulated genes were identified. These genes were enriched in 29 GO terms and 30 KEGG pathways (P ≤ 0.05), including cytokine-cytokine receptor interaction and complement and coagulation cascades, with 17 up-regulated genes and 12 down-regulated genes related to immune responses in the EG relative to the CG. Based on the zebrafish genome, miRNA-seq identified a total of 343 miRNAs, of which 15 were up-regulated and 10 were down-regulated (fold-change ≥2 or ≤0.5 and P ≤ 0.05). Target gene prediction and KEGG enrichment analysis revealed that all of the target genes were concentrated in 13 pathways associated with immune response, including the mTOR signaling pathway and the TGF-beta signaling pathway. The expression patterns of 8 differentially expressed genes and 4 miRNAs involved in immune response were validated by quantitative real-time RT-PCR. These results have provided valuable insights into the molecular mechanisms underlying the immune response of L. longirostris to bacterial haemorrhagic septicemia.
长吻鮠是中国广泛养殖的一种常见鱼类品种，但在养殖过程中，它极易受到嗜水气单胞菌感染引起的细菌性出血败血症的侵害，给养殖户造成巨大的经济损失。为了了解长吻鮠对嗜水气单胞菌感染的免疫反应，本研究采用 Illumina 测序技术分析了脾脏组织中转录组和 microRNA（miRNA）的变化。在本研究中，对照组（CG）和实验组（EG）脾脏样本分别产生了 92.16 和 95.61 百万（M）高质量转录组读数，鉴定出 207 个上调基因和 185 个下调基因。这些基因富集在 29 个 GO 术语和 30 个 KEGG 通路（P ≤ 0.05）中，包括细胞因子-细胞因子受体相互作用和补体与凝血级联，与 CG 相比，EG 中与免疫反应相关的上调基因有 17 个，下调基因有 12 个。基于斑马鱼基因组，miRNA-seq 共鉴定出 343 个 miRNAs，其中 15 个上调，10 个下调（fold-change ≥2 或 ≤0.5，P ≤ 0.05）。靶基因预测和 KEGG 富集分析表明，所有靶基因都集中在 13 个与免疫反应相关的通路中，包括 mTOR 信号通路和 TGF-β 信号通路。通过定量实时 RT-PCR 验证了 8 个差异表达基因和 4 个参与免疫反应的 miRNA 的表达模式。这些结果为长吻鮠对细菌性出血败血症的免疫反应的分子机制提供了有价值的见解。
